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Titolo:
Pathology of fatal West Nile virus infections in native and exotic birds during the 1999 outbreak in New York City, New York
Autore:
Steele, KE; Linn, MJ; Schoepp, RJ; Komar, N; Geisbert, TW; Manduca, RM; Calle, PP; Raphael, BL; Clippinger, TL; Larsen, T; Smith, J; Lanciotti, RS; Panella, NA; McNamara, TS;
Indirizzi:
Wildlife Conservat Soc, Dept Pathol, Bronx, NY 10460 USA Wildlife Conservat Soc Bronx NY USA 10460 ept Pathol, Bronx, NY 10460 USA Wildlife Conservat Soc, Dept Clin Sci, Bronx, NY USA Wildlife Conservat Soc Bronx NY USA at Soc, Dept Clin Sci, Bronx, NY USA USA, Med Res Inst Infect Dis, Div Pathol, Ft Detrick, MD 21702 USA USA FtDetrick MD USA 21702 ect Dis, Div Pathol, Ft Detrick, MD 21702 USA USA, Med Res Inst Infect Dis, Div Virol, Ft Detrick, MD 21702 USA USA Ft Detrick MD USA 21702 fect Dis, Div Virol, Ft Detrick, MD 21702 USA Ctr Dis Control & Prevent, Arbovirus Dis Branch, Div Vector Borne Infect Dis, Natl Ctr Infect Dis, Ft Collins, CO USA Ctr Dis Control & Prevent Ft Collins CO USA fect Dis, Ft Collins, CO USA
Titolo Testata:
VETERINARY PATHOLOGY
fascicolo: 3, volume: 37, anno: 2000,
pagine: 208 - 224
SICI:
0300-9858(200005)37:3<208:POFWNV>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
JAPANESE ENCEPHALITIS-VIRUS; ULTRASTRUCTURE; CELLS;
Keywords:
arbovirus; avian; immunohistochemistry; in situ hybridization; meningoencephalitis; myocarditis; RT-PCR; virus isolation; West Nile virus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: McNamara, TS Wildlife Conservat Soc, Dept Pathol, 2300 So Bldv, Bronx, NY 10460 USA Wildlife Conservat Soc 2300 So Bldv Bronx NY USA 10460 60 USA
Citazione:
K.E. Steele et al., "Pathology of fatal West Nile virus infections in native and exotic birds during the 1999 outbreak in New York City, New York", VET PATH, 37(3), 2000, pp. 208-224

Abstract

West Nile fever caused fatal disease in humans, horses, and birds in the northeastern United States during 1999. We studied birds from two wildlife facilities in New York City, New York, that died or were euthanatized and were suspected to have West Nile virus infections. Using standard histologic and ultrastructural methods, virus isolation, immunohistochemistry, in situhybridization and reverse-transcriptase polymerase chain reaction, we identified West Nile virus as the cause of clinical disease, severe pathologic changes, and death in 27 birds representing eight orders and 14 species. Virus was detected in 23/26 brains (88%), 24/25 hearts (96%), 15/18 spleens (83%), 14/20 Livers (70%), 20/20 kidneys (100%), 10/13 adrenals (77%), 13/14intestines (93%), 10/12 pancreata (83%), 5/12 lungs (42%), and 4/8 ovaries(50%) by one or more methods. Cellular targets included neurons and glial cells in the brain, spinal cord, and peripheral ganglia; myocardial fibers;macrophages and blood monocytes; renal tubular epithelium; adrenal cortical cells; pancreatic acinar cells and islet cells: intestinal crypt epithelium; oocytes; and fibroblasts and smooth muscle cells. Purkinje cells were especially targeted, except in crows and magpies. Gross hemorrhage of the brain, splenomegaly, meningoencephalitis, and myocarditis were the most prominent lesions. Immunohistochemistry was an efficient and reliable method foridentifying infected cases, but the polyclonal antibody cross-reacted withSt. Louis encephalitis virus and other flaviviruses. in contrast, the in situ hybridization probe pWNV-E (WN-USAMRIID99) reacted only with West Nile virus. These methods should aid diagnosticians faced with the emergence of West Nile virus in the United States.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/05/20 alle ore 05:53:37