Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Patterns of protein synthesis and tolerance of anoxia in root tips of maize seedlings acclimated to a low-oxygen environment, and identification of proteins by mass spectrometry
Autore:
Chang, WWP; Huang, L; Shen, M; Webster, C; Burlingame, AL; Roberts, JKM;
Indirizzi:
Univ Calif Riverside, Dept Biochem, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 chem, Riverside, CA 92521 USA Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 2, volume: 122, anno: 2000,
pagine: 295 - 317
SICI:
0032-0889(200002)122:2<295:POPSAT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEHYDROGENASE GENE FAMILY; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; ZEA-MAYS; BETA-GLUCOSIDASE; MESSENGER-RNA; DEPRIVED ROOTS; CYTOPLASMIC PH; EXPRESSION; HYPOXIA; ELECTROPHORESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Roberts, JKM Univ Calif Riverside, Dept Biochem, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 de, CA 92521 USA
Citazione:
W.W.P. Chang et al., "Patterns of protein synthesis and tolerance of anoxia in root tips of maize seedlings acclimated to a low-oxygen environment, and identification of proteins by mass spectrometry", PLANT PHYSL, 122(2), 2000, pp. 295-317

Abstract

Tolerance of anoxia in maize root tips is greatly improved when seedlings are pretreated with 2 to 4 h of hypoxia. We describe the patterns of protein synthesis during hypoxic acclimation and anoxia. We quantified the incorporation of [S-35]methionine into total protein and 262 individual proteins under different oxygen tensions. Proteins synthesized most rapidly under normoxic conditions continued to account for most of the proteins synthesizedduring hypoxic acclimation, while the production of a very few proteins was selectively enhanced. When acclimated root tips were placed under anoxia,protein synthesis was depressed and no "new" proteins were detected. We present evidence that protein synthesis during acclimation, but not during subsequent anoxia, is crucial for acclimation. The complex and quantitative changes in protein synthesis during acclimation necessitate identification of large numbers of individual proteins. We show that mass spectrometry can be effectively used to identify plant proteins arrayed by two-dimensional gel electrophoresis. Of the 48 protein spots analyzed, 46 were identified bymatching to the protein database. We describe the expression of proteins involved in a wide range of cellular functions, including previously reported anaerobic proteins, acid discuss their possible roles in adaptation of plants to low-oxygen stress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:34:25