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Titolo:
Coordinate changes in carbon partitioning and plastidial metabolism duringthe development of oilseed rape embryo
Autore:
Eastmond, PJ; Rawsthorne, S;
Indirizzi:
John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England John InnesCtr Plant Sci Res Norwich Norfolk England NR4 7UH olk, England
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 3, volume: 122, anno: 2000,
pagine: 767 - 774
SICI:
0032-0889(200003)122:3<767:CCICPA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
FATTY-ACID SYNTHESIS; ACETYL-COA CARBOXYLASE; BRASSICA-NAPUS L; ENVELOPE; TRANSPORT; STARCH; BIOSYNTHESIS; ENDOSPERM; MEMBRANE; COENZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Rawsthorne, S John Innes Ctr Plant Sci Res, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res Norwich Res Pk Norwich Norfolk England NR4 7UH
Citazione:
P.J. Eastmond e S. Rawsthorne, "Coordinate changes in carbon partitioning and plastidial metabolism duringthe development of oilseed rape embryo", PLANT PHYSL, 122(3), 2000, pp. 767-774

Abstract

Measurements of metabolic fluxes in whole embryos and isolated plastids have revealed major changes in the pathways of carbon utilization during cotyledon filling by oilseed rape (Brassica napus L.) embryos. In the early cotyledon stage (stage A), embryos used sucrose (Suc) predominantly for starchsynthesis. Plastids isolated from these embryos imported glucose-6-phosphate (Glc-6-P) and partitioned it to starch and fatty acids synthesis and to the oxidative pentose phosphate pathway in the ratio of 2:1:1 on a hexose basis. Of the substrates tested, Glc-6-P gave the highest rates of fatty acid synthesis by the plastids and pyruvate was used weakly. By the mid-to late-cotyledon stage (stage C), oil accumulation by the embryos was rapid, as was their utilization of Suc for oil synthesis in vitro. Plastids from C-stage embryos differed markedly from those of stage-A embryos: (a) pyruvate uptake and utilization for fatty acid synthesis increased by respectively 18- and 25-fold; (b) Glc-6-P partitioning was predominantly to the oxidative pentose phosphate pathway (respective ratios of 1:1:3); and (c) the rate ofplastidial fatty acid synthesis more than doubled. This increased rate of fatty synthesis was dependent upon the increase in pyruvate uptake and was mediated through the induction of a saturable transporter activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:24:17