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Titolo:
Molecular cloning and characterization of an IgE-reactive protein from Anisakis simplex: Ani s 1
Autore:
Arrieta, I; del Barrio, M; Vidarte, L; del Pozo, V; Pastor, C; Gonzalez-Cabrero, J; Cardaba, B; Rojo, M; Minguez, A; Cortegano, I; Gallardo, S; Aceituno, E; Palomino, P; Vivanco, F; Lahoz, C;
Indirizzi:
Fdn Jimenez Diaz, Dept Immunol, E-28040 Madrid, Spain Fdn Jimenez Diaz Madrid Spain E-28040 ept Immunol, E-28040 Madrid, Spain Fdn Jimenez Diaz, Dept Res, E-28040 Madrid, Spain Fdn Jimenez Diaz Madrid Spain E-28040 z, Dept Res, E-28040 Madrid, Spain
Titolo Testata:
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
fascicolo: 2, volume: 107, anno: 2000,
pagine: 263 - 268
SICI:
0166-6851(20000415)107:2<263:MCACOA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALLERGY;
Keywords:
helminth; parasite hypersensitivity; molecular cloning; mRNA; calcium-binding protein; EF-hand; Anisakis simplex; expression library;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Lahoz, C Fdn Jimenez Diaz, Dept Immunol, E-28040 Madrid, Spain Fdn JimenezDiaz Madrid Spain E-28040 ol, E-28040 Madrid, Spain
Citazione:
I. Arrieta et al., "Molecular cloning and characterization of an IgE-reactive protein from Anisakis simplex: Ani s 1", MOL BIOCH P, 107(2), 2000, pp. 263-268

Abstract

Ingestion of the parasitic nematode Anisakis,simplex in undercooked fish can cause severe allergic reactions in some individuals. Using pooled human sera from sensitized patients we have probed an expression library for A. simplex antigens. One positive clone was found to encode a full length 21 kDa protein with strong homology to nematode troponins. The recombinant protein was expressed as a GST-fusion protein and found by immunoblot analysis to react with sera from 20% of allergic patients. The presence of functionalEF-hand Ca2+ binding motifs was demonstrated by gel-shift analysis. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

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Documento generato il 06/04/20 alle ore 07:39:42