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Titolo:
Gene transfer targeting interstitial fibroblasts by the artificial viral envelope-type hemagglutinating virus of Japan liposome method
Autore:
Tsujie, M; Isaka, Y; Ando, Y; Akagi, Y; Kaneda, Y; Ueda, N; Imai, E; Hori, M;
Indirizzi:
Osaka Univ, Grad Sch Med, Dept Internal Med & Therapeut, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 erapeut, Suita, Osaka 5650871, Japan Osaka Univ, Grad Sch Med, Div Gene Therapy Sci, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 apy Sci, Suita, Osaka 5650871, Japan Nara Inst Sci & Technol, Nara 63001, Japan Nara Inst Sci & Technol Nara Japan 63001 ci & Technol, Nara 63001, Japan
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 5, volume: 57, anno: 2000,
pagine: 1973 - 1980
SICI:
0085-2538(200005)57:5<1973:GTTIFB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM CREATININE CONCENTRATION; EPSTEIN-BARR-VIRUS; IN-VIVO; RENAL INJURY; EXPRESSION; GLOMERULONEPHRITIS; KIDNEY; CELLS; BETA; INHIBITION;
Keywords:
progressive renal disease; tubulointerstitial inflammation; fibrosis; gene transfer; lesion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Isaka, Y Osaka Univ, Grad Sch Med, Dept Internal Med & Therapeut, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 5650871, Japan
Citazione:
M. Tsujie et al., "Gene transfer targeting interstitial fibroblasts by the artificial viral envelope-type hemagglutinating virus of Japan liposome method", KIDNEY INT, 57(5), 2000, pp. 1973-1980

Abstract

Background Tubulointerstitial inflammation and fibrosis are commonly associated with most human glomerular diseases. The degree of tubulointerstitialdamage, rather than the glomerular injury, could correlate with the degreeof renal functional impairment and accurately predict long-term prognosis. In an effort to understand the pathogenesis of the progressive interstitial fibrosis, we developed a new strategy of gene transfer to the interstitial fibroblasts. Methods. Either fluorescein isothiocyanate (FITC)-labeled oligodeoxynucleotides (ODNs) or pEBAct-NlacF expression vector was introduced into the kidney of normal rats retrogradely via ureter by using the artificial viral envelope (AVE)type hemagglutinating virus of Japan (HVJ) liposome method. Results. FITC-labeled ODNs were accumulated diffusely in the nuclei of theinterstitial cells in the transfected kidney 10 minutes after transfection, and the interstitial cells were identified as interstitial fibroblasts byimmunostaining with ER-TR7. To examine the gene expression in the interstitium, pEBAct-NlacF gene-conjugated HVJ liposome was injected retrogradely through the ureter, and in consequence, nuclear P-galactosidase activity wascontinuously observed in interstitial cells at least two weeks after transfection. Conclusion. This new strategy of gene transfer to the interstitial fibroblasts is useful for the investigation of the pathophysiology of tubulointerstitial lesion, and furthermore, it may be a promising new therapeutic method for the progression of interstitial fibrosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 21:26:51