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Titolo:
In vivo characterization of renal iron transport in the anaesthetized rat
Autore:
Wareing, M; Ferguson, CJ; Green, R; Riccardi, D; Smith, CP;
Indirizzi:
Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England
Titolo Testata:
JOURNAL OF PHYSIOLOGY-LONDON
fascicolo: 2, volume: 524, anno: 2000,
pagine: 581 - 586
SICI:
0022-3751(20000415)524:2<581:IVCORI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROXIMAL CONVOLUTED TUBULE; POTASSIUM REABSORPTION; ANESTHETIZED RAT; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Wareing, M Univ Manchester, Sch Biol Sci, G38,Stopford Bldg,Oxford Rd, Manchester M139PT, Lancs, England Univ Manchester G38,Stopford Bldg,Oxford Rd Manchester Lancs England M13 9PT
Citazione:
M. Wareing et al., "In vivo characterization of renal iron transport in the anaesthetized rat", J PHYSL LON, 524(2), 2000, pp. 581-586

Abstract

1. In vivo microinjections of (FeCl3)-Fe-55 were made to assess renal iron(Fe2+/3+) transport in the anaesthetized rat.2. Following microinjection into proximal convoluted tubules (PCTs), 18.5 /- 2.9% (mean +/- S.E.M., n = 11) Of the Fe-55 was recovered in the urine. This recovery was not dependent on the injection site indicating that ironis not reabsorbed across the surface convolutions of the proximal tubule.3. Following microinjection into distal convoluted tubules (DCTs) 46.1 +/-6.1% (n = 8) of the injected Fe-55 was recovered. Taken together the recovery data from the PCT and DCT microinjection studies indicate that the transport of iron occurs in the loop of Henle (LH) and collecting duct system.4. In vivo luminal microperfusion was used to examine iron transport by the LR in more detail. In tubules perfused with 7 mu mol l(-1) (FeCl3)-Fe-55,52.7 +/- 8.3% (n = 8) of the perfused Fe-55 was recovered in the collectedfluid, indicating significant iron reabsorption in the LH. Addition of copper (Cu2+ as 7 mu mol l(-1) CuSO4), manganese (Mn2+ as 7 mu mol l(-1) MnSO4) or zinc (Zn2+ as 7 mu mol l(-1) ZnSO4) to the perfusate did not affect reabsorption of water, Na+ or K+, but increased recovery of Fe-55 to 83.5 +/-6.8% (n = 8, P < 0.04), 75.8 +/- 5.9 (n = 6, not significant, n.s.) and 67.9 +/- 3.8; (n = 9, n.s.), respectively.5. Thus, iron transport in the LH can be reduced by the addition of copperor manganese to the luminal perfusate suggesting that these ions may compete with iron for a common transport pathway. However, this pathway may not be shared by zinc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:44:28