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Titolo:
Immunostaining of ganglioside GD1b, GD3 and GM1 in rat cerebellum: Cellular layer and cell type specific associations
Autore:
Molander, M; Berthold, CH; Persson, H; Fredman, P;
Indirizzi:
Univ Gothenburg, Inst Clin Neurosci, Expt Neurosci Sect, Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden xpt Neurosci Sect, Gothenburg, Sweden Univ Gothenburg, Inst Anat & Cell Biol, Neuroanat Sect, Gothenburg, SwedenUniv Gothenburg Gothenburg Sweden l, Neuroanat Sect, Gothenburg, Sweden
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 4, volume: 60, anno: 2000,
pagine: 531 - 542
SICI:
0360-4012(20000515)60:4<531:IOGGGA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; MONOCLONAL-ANTIBODIES; ULTRASTRUCTURAL-LOCALIZATION; IMMUNOELECTRON MICROSCOPY; MONOSIALOGANGLIOSIDE GM1; CULTURED NEURONS; PURKINJE-CELLS; HUMAN-BRAIN; GLYCOSPHINGOLIPIDS; EXPRESSION;
Keywords:
brain; immunohistochemistry; glycosphingolipids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Molander, M Univ Gothenburg, Inst Clin Neurosci, Expt Neurosci Sect, Sahlgrenska Univ Hosp, SE-43180 Molndal, Sweden Univ Gothenburg Molndal SwedenSE-43180 3180 Molndal, Sweden
Citazione:
M. Molander et al., "Immunostaining of ganglioside GD1b, GD3 and GM1 in rat cerebellum: Cellular layer and cell type specific associations", J NEUROSC R, 60(4), 2000, pp. 531-542

Abstract

We have studied the cellular distribution of gangliosides GD1b, GD3 and GM1 in rat cerebellum by immunostaining, using monoclonal antibodies and confocal microscopy. Antibodies against astroglial, neuronal and synaptic vesicle associated molecules were used for colocalization analyses. In the gray matter, the anti-GD1b antibody stained thin strands in the molecular layer (ML), interpreted as Bergman glia fibers based on colocalized staining withanti-glial fibrillary acidic protein (GFAP), The neuropil in the granule (GL) and Purkinje (PL) cell layers was also anti-GD1b positive. The anti-GD3antibody stained the ML, the neuropil in the GL and PL and also the granule and Purkinje cell bodies, appearing intracytoplasmically and vesicle associated. Anti-GD1b and anti-GD3 staining in the GL glomeruli were colocalized with anti-synaptophysin staining. The anti-GM1 antibody stained cell bodies in the ML but they could not be characterized in colocalization experiments. The GL and PL were not stained with the anti-GM1 antibody. In the white matter, different staining patterns were seen for the gangliosides, the anti-GM1 staining being the most intense. This study shows cellular layer and cell type specific associations of the investigated gangliosides and localization of GD1b and GD3 at synaptic sites, warranting further studies on their role in synaptic mechanisms. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:32:28