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Titolo:
A two-vector system for the production of recombinant polyketides in Streptomyces
Autore:
Ziermann, R; Betlach, MC;
Indirizzi:
Kosan Biosci Inc, Hayward, CA 94545 USA Kosan Biosci Inc Hayward CA USA 94545 n Biosci Inc, Hayward, CA 94545 USA
Titolo Testata:
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
fascicolo: 1, volume: 24, anno: 2000,
pagine: 46 - 50
SICI:
1367-5435(200001)24:1<46:ATSFTP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENGINEERED BIOSYNTHESIS; INTEGRATIVE VECTORS; CLONING VECTORS; SYNTHASES; GENES; HOST; DNA;
Keywords:
polyketide; Streptomyces; erythromycin; shuttle vector;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Betlach, MC Kosan Biosci Inc, 3832 Bay Ctr Pl, Hayward, CA 94545 USA KosanBiosci Inc 3832 Bay Ctr Pl Hayward CA USA 94545 4545 USA
Citazione:
R. Ziermann e M.C. Betlach, "A two-vector system for the production of recombinant polyketides in Streptomyces", J IND MIC B, 24(1), 2000, pp. 46-50

Abstract

A two-vector system was developed for heterologous expression of the threegenes comprising the 6-deoxyerythronolide B synthase (DEBS) polyketide gene cluster. Individual DEBS genes and pairwise combinations of two such genes were each cloned downstream of the actinorhodin (actI) promoter in two compatible Streptomyces vectors: the autonomously replicating vector, pKAO127'Kan', and the integrating vector, pSET152, The resulting plasmids were either simultaneously or sequentially transformed into Streptomyces lividans K4-114. Efficient trans-complementation of modular polyketide synthase subunit proteins occurred when the respective genes were transcribed from the two vectors and resulted in production of the erythromycin precursor 6-deoxyerythronolide B (6-dEB).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 03:10:31