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Titolo:
Induction of an antigen-specific CTL response by a conformationally biasedagonist of human C5a anaphylatoxin as a molecular adjuvant
Autore:
Ulrich, JT; Cieplak, W; Paczkowski, NJ; Taylor, SM; Sanderson, SD;
Indirizzi:
Univ Nebraska, Med Ctr 986805, Eppley Inst Res Canc & Allied Dis, Omaha, NE 68198 USA Univ Nebraska Omaha NE USA 68198 s Canc & Allied Dis, Omaha, NE 68198 USA Corixa, Hamilton, MT 59840 USA Corixa Hamilton MT USA 59840Corixa, Hamilton, MT 59840 USA Univ Queensland, Dept Physiol & Pharmacol, St Lucia, Qld, Australia Univ Queensland St Lucia Qld Australia armacol, St Lucia, Qld, Australia
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 10, volume: 164, anno: 2000,
pagine: 5492 - 5498
SICI:
0022-1767(20000515)164:10<5492:IOAACR>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MHC CLASS-I; BACTERIAL TOXINS; PROTEOLYTIC ACTIVATION; DECAPEPTIDE AGONISTS; EFFECTOR REGION; COMPLEMENT; RECEPTOR; IMMUNITY; PEPTIDE; PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Sanderson, SD Univ Nebraska, Med Ctr 986805, Eppley Inst Res Canc & AlliedDis, 600 S 42nd St, Omaha, NE 68198 USA Univ Nebraska 600 S 42nd St Omaha NE USA 68198 NE 68198 USA
Citazione:
J.T. Ulrich et al., "Induction of an antigen-specific CTL response by a conformationally biasedagonist of human C5a anaphylatoxin as a molecular adjuvant", J IMMUNOL, 164(10), 2000, pp. 5492-5498

Abstract

A conformationally biased decapeptide agonist of human C5a anaphylatoxin (YSPKPMPLaR) was used as a molecular adjuvant in stimulating an Ag-specific CTL response against murine P815S target cells expressing an Ld-restricted CTL epitope of the hepatitis B surface Ag (HBsAg), Groups of BALB/c mice (H-2(d)) were immunized with aqueous solutions of the HBsAg CTL epitopes (IPQSLDSWWTSL and IPQSLDSTaVTSLRR); the C5a agonist (YSFKPMPLaR); the C5a agonist and HBsAg CTL epitopes admired (IPQSLDSWWTSL and IPQSLDSWWTSLRR + YSFKPMPLaR); the C5a-active, HBsAg CTL epitope-C5a agonist constructs (IPQSLDSWWTSLYSFKPMPLaR, IPQSLDSWWTSLRRYSFKPMPLaR, and IPQSLDSWWTSLRVRRYSFPMPLaR); a C5a-inactive, reverse-moiety construct (YSFKPMPLaRRRIPQSLDSWWTSL); and a C5a-attenuated, carboxyl-terminal-blocked construct (IPQSLDSWWTSLRRYSFKPMPLaRG). Ag-specific CD8(+) CTL responses were observed after the secondary boostin the absence of any added adjuvant only in mice that were immunized withC5a-active contructs, IPQSLDSWWTSLRRYSFKPMPLaR and IPQSLDSWWTSLRVRRYSFKPMPLaR. These two C5a-active immunogens contained potential subtilisin-sensitive linker sequences between the HBsAg CTL epitope and the C5a agonist; i.e., a double-Arg (RR) and a furin protease sensitive sequence (RVRR), The introduction of these potentially cleavable sequences may be a method of increasing the likelihood of liberating the CTL epitope from the C5a agonist by intracellular proteases, thereby facilitating entry of the epitope into Ag-processing pathways via an exogenous route.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 21:52:47