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Titolo:
A new look at Syk in alpha beta and gamma delta T cell development using chimeric mice with a low competitive hematopoietic environment
Autore:
Colucci, F; Guy-Grand, D; Wilson, A; Turner, M; Schweighoffer, E; Tybulewicz, VLJ; Di Santo, JP;
Indirizzi:
Inst Pasteur, Lab Cytokines & Lymphoid Dev, F-75015 Paris, France Inst Pasteur Paris France F-75015 & Lymphoid Dev, F-75015 Paris, France Hop Necker Enfants Malad, INSERM, U429, Paris, France Hop Necker Enfants Malad Paris France alad, INSERM, U429, Paris, France Ludwig Inst Canc Res, Eplainger, Switzerland Ludwig Inst Canc Res Eplainger Switzerland Res, Eplainger, Switzerland Babraham Inst, Cambridge, England Babraham Inst Cambridge EnglandBabraham Inst, Cambridge, England Natl Inst Med Res, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA ed Res, London NW7 1AA, England
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 10, volume: 164, anno: 2000,
pagine: 5140 - 5145
SICI:
0022-1767(20000515)164:10<5140:ANLASI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE KINASE; POSITIVE SELECTION; EXPRESSION; ZAP-70; DIFFERENTIATION; THYMOCYTES; TCR; FAMILY; REARRANGEMENT; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Colucci, F Inst Pasteur, Lab Cytokines & Lymphoid Dev, 25 Rue Dr Roux, F-75015 Paris,France Inst Pasteur 25 Rue Dr Roux Paris France F-75015 Paris,France
Citazione:
F. Colucci et al., "A new look at Syk in alpha beta and gamma delta T cell development using chimeric mice with a low competitive hematopoietic environment", J IMMUNOL, 164(10), 2000, pp. 5140-5145

Abstract

The Syk protein tyrosine kinase (PTK) is essential for B, but not T or NK,cell development, although certain T cell subsets (i.e., gamma delta T cells of intestine and skin) appear to be dependent on Syk, In this report, wehave re-evaluated the role of Syk in T cell development in hematopoietic chimeras generated by using Syk-deficient fetal liver hematopoietic stem cells (FL-HSC), We found that Syk(-/-) FL-HSC were vastly inferior to wild-type FL-HSC in reconstituting T cell development in recombinant-activating gene 2 (RAG2)-deficient mice, identifying an unexpected and nonredundant role for Syk in this process. This novel function of Syk in T cell development was mapped to the CD44(-)CD25(+) stage. According to previous reports, development of intestinal gamma delta T cells was arrested in Syk(-/- -)-->RAG2(-/-) chimeras, In striking contrast, when hosts were the newly established alymphoid RAG2 x common cytokine receptor gamma-chain (RAG2/gamma(c)) mice,Syk(-/-) chimeras developed intestinal gamma delta T cells as well as other T cell subsets (including alpha beta T cells, NK1.1(+) alpha beta T cells, and splenic and thymic gamma delta T cells). However, all Syk-deficient Tcell subsets were reduced in number, reaching about 25-50% of controls. These results attest to the utility of chimeric mice generated in a low competitive hematopoietic environment to evaluate more accurately the impact of lethal mutations on lymphoid development. Furthermore, they suggest that Syk intervenes in early T cell development independently of ZAP-70, and demonstrate that Syk is not essential for the intestinal gamma delta T cell lineage to develop.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 21:53:49