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Titolo:
FAS LIGAND (CD95L) AND B7 EXPRESSION ON DENDRITIC CELLS PROVIDE COUNTER-REGULATORY SIGNALS FOR T-CELL SURVIVAL AND PROLIFERATION
Autore:
LU LN; QIAN SG; HERSHBERGER PA; RUDERT WA; LYNCH DH; THOMSON AW;
Indirizzi:
UNIV PITTSBURGH,MED CTR,THOMAS E STARZL TRANSPLANT INST,W1544 FLOOR,BIOMED SCI TOWER PITTSBURGH PA 15213 UNIV PITTSBURGH,MED CTR,THOMAS E STARZL TRANSPLANT INST PITTSBURGH PA15213 UNIV PITTSBURGH,DEPT SURG PITTSBURGH PA 15213 UNIV PITTSBURGH,DEPT MOL GENET & BIOCHEM PITTSBURGH PA 15213 UNIV PITTSBURGH,DEPT PEDIAT PITTSBURGH PA 15213 IMMUNEX RES & DEV CORP SEATTLE WA 98101
Titolo Testata:
The Journal of immunology
fascicolo: 12, volume: 158, anno: 1997,
pagine: 5676 - 5684
SICI:
0022-1767(1997)158:12<5676:FL(ABE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; ACTIVATION-INDUCED APOPTOSIS; BONE-MARROW; DNA FRAGMENTATION; GRAFT ACCEPTANCE; DEATH; TOLERANCE; LYMPHOCYTES; MICE; PROGENITORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
L.N. Lu et al., "FAS LIGAND (CD95L) AND B7 EXPRESSION ON DENDRITIC CELLS PROVIDE COUNTER-REGULATORY SIGNALS FOR T-CELL SURVIVAL AND PROLIFERATION", The Journal of immunology, 158(12), 1997, pp. 5676-5684

Abstract

Activation of T cells is induced efficiently by dendritic cells (DC),but little is known about the role of DC in the regulation of T cell death, In this study, highly purified DC (DEC-205(+), MHC class Il(high), B7-1(+) [CD80(+)], B7-2(high) [CD86(high)], CD40(+), CD11c(+)) grown from normal mouse bone marrow in granulocyte-macrophage CSF + IL-4 were found to express FasL (CD95L) mRNA by reverse transcriptase PCR and to uniformly express Fast by both flow cytometric and immunocytochemical analyses. These cells, but not DC propagated from FasL-deficient(Bb,gld) mice, induced dose-dependent increases in DNA fragmentation in Fas(+) Jurkat T cells over 18 h coculture, Addition of mouse Fas-Fcfusion protein at the start of the cultures diminished this effect. Even at high relative concentrations, however, B7-2(high) DC induced only low levels of DNA fragmentation in Con A or alloactivated splenic Tcells, as determined by radio- or spectrofluorometric assays and by in situ nick-end labeling, However, in the presence of CTLA4lg, a molecule that blocks the B7-CD28 costimulatory pathway, DC that failed to stimulate in primary MLR induced markedly augmented levels of apoptosisin alloactivated T cells, CTLA4lg treatment also increased the level of DNA fragmentation induced by FasL-deficient DC, indicating the existence of additional potential (Fas-independent) pathways of DC-inducedT cell death, These findings suggest that the costimulatory (B7-CD28)and T cell death-inducing pathways may play important counter-regulatory roles in dictating the outcome of (allogeneic) DC-T cell interactions.

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Documento generato il 19/09/20 alle ore 15:25:41