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Titolo:
Complement activation induced by ischemia-reperfusion in humans: a study in patients undergoing partial hepatectomy
Autore:
Straatsburg, IH; Boermeester, MA; Wolbink, GJ; van Gulik, TM; Gouma, DJ; Frederiks, WM; Hack, CE;
Indirizzi:
Univ Amsterdam, Surg Lab IWO1 155, Dept Cell Biol & Histol, Acad Med Ctr, NL-1105 AZ Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1105 AZ Z Amsterdam, Netherlands Univ Amsterdam, Acad Med Ctr, Dept Surg, NL-1105 AZ Amsterdam, NetherlandsUniv Amsterdam Amsterdam Netherlands NL-1105 AZ Z Amsterdam, Netherlands Netherlands Red Cross, Blood Transfus Serv, Cent Lab, Amsterdam, Netherlands Netherlands Red Cross Amsterdam Netherlands Lab, Amsterdam, Netherlands
Titolo Testata:
JOURNAL OF HEPATOLOGY
fascicolo: 5, volume: 32, anno: 2000,
pagine: 783 - 791
SICI:
0168-8278(200005)32:5<783:CAIBII>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-REACTIVE PROTEIN; ACUTE MYOCARDIAL-INFARCTION; CORONARY-ARTERY OCCLUSION; LIVER-TRANSPLANTATION; HEPATIC ISCHEMIA; SKELETAL-MUSCLE; INJURY; COMPLEXES; NEUTROPHILS; EXPRESSION;
Keywords:
C3; C4; classical complement pathway; C-reactive protein; liver resection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Straatsburg, IH Univ Amsterdam, Surg Lab IWO1 155, Dept Cell Biol & Histol, Acad Med Ctr, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands Univ Amsterdam Meibergdreef 9 Amsterdam Netherlands NL-1105 AZ
Citazione:
I.H. Straatsburg et al., "Complement activation induced by ischemia-reperfusion in humans: a study in patients undergoing partial hepatectomy", J HEPATOL, 32(5), 2000, pp. 783-791

Abstract

Background/Aim: Activation of the complement system is induced by ischemia-reperfusion (IIR) in animal models. Whether I/R also induces complement activation in humans is not known. Here, we investigated complement activation in patients undergoing major liver resection,Methods: In 11 of 17 patients, the hepatoduodenal ligament was clamped, making the liver transiently ischemic (HEMI+; mean ischemia time, 42+/-18 min); 6 patients were operated without clamping (HEMI-). Activation at plasma level (circulating activation products) was studied in blood samples collected prior to surgery and 5, 24 and 48 h thereafter. Parameters analyzed were C4b/c and C3b/c, C4d and C3d, C3a, as well as complexes between complement and C-reactive protein (CRP), which reflect CRP-induced complement activation. Activation at tissue level (C3 and C4 fixation) was studied in liver biopsies obtained before and after resection,Results: In plasma, post-operative levels of C4b/c and C3b/c were not different from baseline levels in both groups. Mean plasma levels of C4b/c and C3b/c were significantly decreased at 24 h post-surgery in the HEMI+ group (p=0.02 and p=0.07), At the same time, levels of C4d-CRP and C3d-CRP were significantly increased (p<0.01 for both parameters). At tissue level, activated complement fragments were observed intracellularly in some pericentralhepatocytes. In I/R livers, large numbers of hepatocytes were positively stained for an complement activation products. Conclusions: Our data show that in situ complement activation via the classical route occurred during liver resection and that ischemia and/or reperfusion may have contributed to activation. Levels of complement activation products in the circulation were low, showing that transient ischemia had nosevere influence on systemic complement activation, suggesting a locally contained response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 13:57:37