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Titolo:
Inhibition of CD83 cell surface expression during dendritic cell maturation by interference with nuclear export of CD83 mRNA
Autore:
Kruse, M; Rosorius, O; Kratzer, F; Bevec, D; Kuhnt, C; Steinkasserer, A; Schuler, G; Hauber, J;
Indirizzi:
Univ Erlangen Nurnberg, Inst Clin & Mol Virol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Univ Erlangen Nurnberg, Dept Dermatol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Novartis Res Inst, Dept Immunol, A-1235 Vienna, Austria Novartis Res InstVienna Austria A-1235 Immunol, A-1235 Vienna, Austria
Titolo Testata:
JOURNAL OF EXPERIMENTAL MEDICINE
fascicolo: 9, volume: 191, anno: 2000,
pagine: 1581 - 1589
SICI:
0022-1007(20000501)191:9<1581:IOCCSE>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN DEOXYHYPUSINE SYNTHASE; EUKARYOTIC INITIATION FACTOR-5A; PROTEIN-SYNTHESIS; NUCLEOCYTOPLASMIC TRANSPORT; POSTTRANSLATIONAL FORMATION; LANGERHANS CELLS; FACTOR EIF-5A; HUMAN BLOOD; HELA-CELLS; FACTOR 5A;
Keywords:
dendritic cells; CD83; hypusine; eIF-5A; nuclear export;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Hauber, J Univ Erlangen Nurnberg, Inst Clin & Mol Virol, Schlossgarten 4, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Schlossgarten 4 Erlangen Germany D-91054
Citazione:
M. Kruse et al., "Inhibition of CD83 cell surface expression during dendritic cell maturation by interference with nuclear export of CD83 mRNA", J EXP MED, 191(9), 2000, pp. 1581-1589

Abstract

Dendritic cells (DCs), nature's adjuvant, must mature to sensitize T cells. However, although the maturation process is essential, it is not yet fully understood at thr molecular level. In this study, we investigated the course of expression of the unique hypusine-containing protein eukaryotic initiation factor 5A (eIF-5A), which is part of a particular RNA nuclear exportpathway, during in vitro generation of human DCs. We show that eIF-5A expression is significantly upregulated during DC maturation. Furthermore, an inhibitor of the hypusine modification, GC7 (N-1-guanyl-1,7-diaminoheptane),prevents CD83 surface expression by apparently interfering with nucleocytoplasmic translocation of the CD83 mRNA and, importantly, significantly inhibits DC-mediated T lymphocyte activation. The data presented suggest that CD83 mRNA is transported from the nucleus to the cytoplasm via a specific nuclear export pathway and that hypusine formation appears to be essential for the maturation of functional DCs. Therefore, pharmacological interferencewith hypusine formation may provide a new possibility to modulate DC function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 21:21:51