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Titolo:
Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections
Autore:
Martin, DA; Muth, DA; Brown, T; Johnson, AJ; Karabatsos, N; Roehrig, JT;
Indirizzi:
Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Natl Ctr Infect Dis, Publ Hlth Serv,US Dept Hlth & Human Serv, Ft Collins, CO 80522 USA Ctr Dis Control & Prevent Ft Collins CO USA 80522 t Collins, CO 80522 USA
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 5, volume: 38, anno: 2000,
pagine: 1823 - 1826
SICI:
0095-1137(200005)38:5<1823:SOIMCE>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODIES; LOUIS ENCEPHALITIS; VIRUS; IDENTIFICATION; HUMANS; SERODIAGNOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Martin, DA Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Natl Ctr Infect Dis, Publ Hlth Serv,US Dept Hlth & Human Serv, POB 2087, Ft Collins, CO 80522USA Ctr Dis Control & Prevent POB 2087 Ft Collins CO USA 80522 2USA
Citazione:
D.A. Martin et al., "Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections", J CLIN MICR, 38(5), 2000, pp. 1823-1826

Abstract

Immonoglobulin M antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) is a rapid and versatile diagnostic method that readily permits the combination of multiple assays, Test consolidation is especially important for arthropod-borne viruses (arboviruses) which belong to at least three virus families: the Togaviridae, Flaviviridae, and Bunyaviridae. Using prototype viruses from each of these families and a panel of well-characterized human sera, we have evaluated and standardized a combined MAC-ELISA capable of identifying virus infections caused by members of each virus family, Furthermore, by grouping antigens geographically and utilizing known serological cross-reactivities, we have reduced the number of antigens necessary for testing, while maintaining adequate detection sensitivity. We have determined that a 1:400 serum dilution is most appropriate for screening antiviral antibody, using a positive-to-negative ratio of greater than or equal to 2.0 as a positive cutoff value. With a blind-ceded human serum panel, this combined MAC-ELISA was shown to have test sensitivity and specificity that correlated well with those of other serological techniques.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 06:40:35