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Titolo:
Bidirectional transcytosis of IgG by the rat neonatal Fc receptor expressed in a rat kidney cell line: a system to study protein transport across epithelia
Autore:
McCarthy, KM; Yoong, Y; Simister, NE;
Indirizzi:
Brandeis Univ, WM Keck Inst Cellular Visualizat, Rosenstiel Ctr Basic Biomed Sci, Waltham, MA 02254 USA Brandeis Univ Waltham MA USA 02254 asic Biomed Sci, Waltham, MA 02254 USA Brandeis Univ, Dept Biol, Waltham, MA 02254 USA Brandeis Univ Waltham MA USA 02254 Univ, Dept Biol, Waltham, MA 02254 USA
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 7, volume: 113, anno: 2000,
pagine: 1277 - 1285
SICI:
0021-9533(200004)113:7<1277:BTOIBT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERIC IMMUNOGLOBULIN RECEPTOR; PLASMA-MEMBRANE PROTEINS; I-RELATED RECEPTOR; MDCK CELLS; PHOSPHATIDYLINOSITOL 3-KINASE; HUMAN PLACENTA; SMALL-INTESTINE; TRANSEPITHELIAL TRANSPORT; PHOSPHOINOSITIDE 3-KINASE; INHIBITS TRANSCYTOSIS;
Keywords:
FcRn; transcytosis; cell polarity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Simister, NE Brandeis Univ, WM Keck Inst Cellular Visualizat, Rosenstiel Ctr Basic Biomed Sci, Waltham, MA 02254 USA Brandeis Univ Waltham MA USA 02254 ci, Waltham, MA 02254 USA
Citazione:
K.M. McCarthy et al., "Bidirectional transcytosis of IgG by the rat neonatal Fc receptor expressed in a rat kidney cell line: a system to study protein transport across epithelia", J CELL SCI, 113(7), 2000, pp. 1277-1285

Abstract

The neonatal Fc receptor, FcRn, transports immunoglobulin G (IgG) across cellular barriers between mother and offspring. FcRn also protects circulating IgG from catabolism, probably during transport across the capillary endothelium, Only one cell culture model of transcytosis has been used extensively, the transport of IgA from the basolateral to the apical surface of Madin-Darby canine kidney cells by the polymeric immunoglobulin receptor (pIgR), We report that rat inner medullary collecting duct (IMCD) cells transfected with DNA encoding the a subunit of rat FcRn specifically and saturably transport Fc when grown as polarized monolayers, Using this system, we havefound that transcytosis by FcRn, like transcytosis by the pIgR, depends upon an intact microtubule system. FcRn differs most strikingly from the pIgRin its ability to transport its ligand in both the apical to basolateral and basolateral to apical directions, The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibited basolateral to apical transport byFcRn more than apical to basolateral transport, suggesting that there are differences in the mechanisms of transport in the two directions. Lastly, we found that transcytosis by FcRn depends upon vesicular acidification. We anticipate that the IMCD cell culture model will allow further elucidation of the mechanism of IgG transport by FcRn.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 00:54:34