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Titolo:
Actin filaments and microtubules play different roles during bristle elongation in Drosophila
Autore:
Tilney, LG; Connelly, PS; Vranich, KA; Shaw, MK; Guild, GM;
Indirizzi:
Univ Penn, Dept Biol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Dept Biol, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 7, volume: 113, anno: 2000,
pagine: 1255 - 1265
SICI:
0021-9533(200004)113:7<1255:AFAMPD>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SHAPE CHANGES; F-ACTIN; TISSUE POLARITY; MORPHOGENESIS; CYTOSKELETON; ORGANIZATION; PHALLOIDIN; ENCODES; BINDING; GTPASES;
Keywords:
actin; Drosophila; bristle; bundle assembly; regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Guild, GM Univ Penn, Dept Biol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Philadelphia, PA 19104 USA
Citazione:
L.G. Tilney et al., "Actin filaments and microtubules play different roles during bristle elongation in Drosophila", J CELL SCI, 113(7), 2000, pp. 1255-1265

Abstract

Developing bristles in Drosophila pupae contain 7-11 bundles of crosslinked actin filaments and a large population of microtubules, During bristle growth the rate of cell elongation increases with bristle length. Thin section EM shows that bundle size is correlated with the amount of cytoplasm at all points along the bristle. Thus, as the bristle elongates and tapers, fewer actin filaments are used. To ensure penetration of inhibitors we isolated thoraces and cultured them in vitro; bristles elongate at rates identicalto bristles growing in situ, Interestingly, inhibitors of actin filament assembly (cytochalasin D and latrunculin A) dramatically curtailed bristle elongation while a filament stabilizer (jasplakinolide) accelerated elongation. In contrast, inhibitors of microtubule dynamics (nocodazole, vinblastine, colchicine and taxol) did not affect bristle elongation. Surprisingly, the bristle microtubules are stable and do not turn over. Furthermore, the density of microtubules decreases as the bristle elongates. These two facts coupled with calculations and kinetics of elongation and the fact that the microtubules are short indicate that the microtubules are assembled early in development and then transported distally as the bristle grows. We conclude that actin assembly is crucial for bristle cell elongation and that microtubules must furnish other functions such as to provide bulk to the bristle cytoplasm as well as playing a role in vesicle transport.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 03:53:13