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Titolo:
A checkpoint that monitors cytokinesis in Schizosaccharomyces pombe
Autore:
Liu, JH; Wang, HY; Balasubramanian, MK;
Indirizzi:
Natl Univ Singapore, Inst Mol Agrobiol, Cell Div Lab, Singapore 117604, Singapore Natl Univ Singapore Singapore Singapore 117604 ngapore 117604, Singapore
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 7, volume: 113, anno: 2000,
pagine: 1223 - 1230
SICI:
0021-9533(200004)113:7<1223:ACTMCI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOSIN HEAVY-CHAIN; SPINDLE POLE BODY; FISSION YEAST; SEPTUM FORMATION; PROTEIN-KINASE; ACTOMYOSIN RING; DIVISION SEPTUM; CELL-DIVISION; F-ACTIN; GENE;
Keywords:
cytokinesis; checkpoint; Schizosaccharomyces pombe; Cps1p;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Balasubramanian, MK Natl Univ Singapore, Inst Mol Agrobiol, Cell Div Lab, 1 Res Link, Singapore 117604, Singapore Natl Univ Singapore 1 Res Link Singapore Singapore 117604
Citazione:
J.H. Liu et al., "A checkpoint that monitors cytokinesis in Schizosaccharomyces pombe", J CELL SCI, 113(7), 2000, pp. 1223-1230

Abstract

Cell division in Schizosaccharomyces pombe is achieved through the use of a medially positioned actomyosin ring. A division septum is formed centripetally, concomitant with actomyosin ring constriction. Genetic screens have identified mutations in a number of genes that affect actomyosin ring or septum assembly. These cytokinesis-defective mutants, however, undergo multiple S and M phases and die as elongated cells with multiple nuclei, Recently; we have shown that a mutant allele of the S, pombe drc1(+)/cps1(+) gene, which encodes a 1,3-beta-glucan synthase subunit? is defective in cytokinesis but displays a novel phenotype. drc1-191/cps1-191 cells are capable of assembling actomyosin rings and completing mitosis, but are incapable of assembling the division septum, causing them to arrest as binucleate cells with a stable actomyosin ring. Each nucleus in arrested cps1-191 cells is ableto undergo S phase but these G(2) nuclei are significantly delayed for entry into the hi phase. In this study me have investigated the mechanism thatcauses cps1-191 to block with two G(2) nuclei. We show that the inability of cps1-191 mutants to proceed through multiple mitotic cycles is not related to a defect in cell growth. Rather, the failure to complete some aspect of cytokinesis mag prevent the G(2)/M transition of the two interphase-G(2)nuclei. The G(2)/M transition defect of cps1-191 mutants is suppressed by a mutation in the wee1 gene and also by the dominant cdc2 allele cdc2-1w, but not the cdc2-3w allele, Transient depolymerization of all F-actin structures also allowed a significant proportion of the cps1-191 cells to undergoa second round of mitosis. We conclude that an F-actin and Wee1p dependentcheckpoint blocks G(2)/M transition until previous cytokinesis is completed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 01:31:39