Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Endomorphins: Localization, release and action on rat dorsal horn neurons
Autore:
Dun, NJ; Dun, SL; Wu, SY; Williams, CA; Kwok, EH;
Indirizzi:
E Tennessee State Univ, Quillen Dishner Coll Med, Dept Pharmacol, Johnson City, TN 37614 USA E Tennessee State Univ Johnson City TN USA 37614 hnson City, TN 37614 USA E Tennessee State Univ, Quillen Dishner Coll Med, Dept Physiol, Johnson City, TN 37614 USA E Tennessee State Univ Johnson City TN USA 37614 hnson City, TN 37614 USA
Titolo Testata:
JOURNAL OF BIOMEDICAL SCIENCE
fascicolo: 3, volume: 7, anno: 2000,
pagine: 213 - 220
SICI:
1021-7770(200005/06)7:3<213:ELRAAO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSTANTIA-GELATINOSA NEURONS; SPINAL-CORD; OPIOID RECEPTORS; IMMUNOREACTIVITY; INHIBITION; MONKEY; FIBERS; BRAIN;
Keywords:
mu-opiate receptors; excitatory postsynaptic currents; inhibitory postsynaptic currents;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Dun, NJ E Tennessee State Univ, Quillen Dishner Coll Med, Dept Pharmacol, POB 70577, Johnson City, TN 37614 USA E Tennessee State Univ POB 70577 Johnson City TN USA 37614 614 USA
Citazione:
N.J. Dun et al., "Endomorphins: Localization, release and action on rat dorsal horn neurons", J BIOMED SC, 7(3), 2000, pp. 213-220

Abstract

Endomorphin (Endo) 1 and 2, two tetrapeptides isolated from the bovine andhuman brain, have been proposed to be the endogenous ligand for the mu-opiate receptor. A multi-disciplinary study was undertaken to address the issues of localization, release and biological action of Endo with respect to the rat dorsal horn. First, immunohistochemical studies showed that Endo-1- or Endo-2-like immunoreactivity (Endo-1- or Endo-2-LI) is selectively expressed in fiber-like elements occupying the superficial layers of the rat dorsal horn, which also exhibit a high level of mu-opiate receptor immunoreactivity. Second, release of immunoreactive Endo-2-like substances (irEndo) from the in vitro rat spinal cords upon electrical stimulation of dorsal rootafferent fibers was detected by the immobilized antibody microprobe technique. The site of release corresponded to laminae I and II where the highestdensity of Endo-2-LI fibers was localized. Lastly, whole-cell patch damp recordings from substantia gelatinosa (SG) neurons of rat lumbar spinal cordslices revealed two distinct actions of exogenous Endo-1 and Endo-2: (1) depression of excitatory and/or inhibitory postsynaptic potentials evoked bystimulation of dorsal root entry zone, and (2) hyperpolarization of SG neurons. These two effects were prevented by the selective mu-opiate receptor antagonist beta-funaltrexamine. The localization of endomorphin-positive fibers in superficial layers of the dorsal horn and the release of irEndo upon stimulation of dorsal root afferents together with the observation that Endo inhibits the activity of SG neurons by interacting with mu-opiate receptors provide additional support of a role of Endo as the endogenous ligand for the mu-opiate receptor in the rat dorsal horn. Copyright (C) 2000 National Science Council, ROC and S. Karger AG. Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:22:14