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Titolo:
Infection of organotypic slice cultures from rat central nervous tissue with Trypanosoma brucei brucei
Autore:
Stoppini, L; Buchs, PA; Brun, R; Muller, D; Duport, S; Parisi, L; Seebeck, T;
Indirizzi:
Univ Bern, Inst Gen Microbiol, CH-3012 Bern, Switzerland Univ Bern Bern Switzerland CH-3012 Microbiol, CH-3012 Bern, Switzerland Ctr Med Univ Geneva, Dept Pharmacol, CH-1211 Geneva, Switzerland Ctr Med Univ Geneva Geneva Switzerland CH-1211 -1211 Geneva, Switzerland Swiss Trop Inst, CH-4051 Basel, Switzerland Swiss Trop Inst Basel Switzerland CH-4051 st, CH-4051 Basel, Switzerland
Titolo Testata:
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
fascicolo: 1, volume: 290, anno: 2000,
pagine: 105 - 113
SICI:
1438-4221(200003)290:1<105:IOOSCF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL AFRICAN TRYPANOSOMIASIS; SLEEPING SICKNESS; MICE; SYSTEM; GAMBIENSE; BRAIN; RHODESIENSE; ACTIVATION; MENINGOENCEPHALITIS; IMMUNOPATHOLOGY;
Keywords:
organotypic slice culture; African trypanosomes; nervous tissue; CNS infection; sleeping sickness;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Seebeck, T Univ Bern, Inst Gen Microbiol, Beltzerstr 4, CH-3012 Bern, Switzerland Univ Bern Beltzerstr 4 Bern Switzerland CH-3012 n, Switzerland
Citazione:
L. Stoppini et al., "Infection of organotypic slice cultures from rat central nervous tissue with Trypanosoma brucei brucei", INT J MED M, 290(1), 2000, pp. 105-113

Abstract

We recently described a new procedure to grow nervous tissue as organotypic culture. The main feature of these slice cultures is to maintain a well preserved, three-dimensional organisation of the central nervous tissue. As these cultures can be kept for several weeks (up to three months), we have used this in vitro approach to study the complex interactions between host tissue and parasites during late stages of cerebral African trypanosomiasis. Light and electron microscopical studies, as well as electrophysiologicalrecordings demonstrate that the structure and function of the nervous tissue is not severely affected even after several weeks of trypanosome infection. The presence of a large number of parasites does not seem to be deleterious to neuronal survival. Secondly, most of the trypanosomes are located around the periphery of the nervous tissue, but many of them also penetrate into the nervous parenchyma. Thirdly, trypanosomes with well-conserved morphology are found within the cytoplasm of glial cells, which in some cases were identified as astrocytes. These "intracellular parasites" seem to actively invade the target cells. Our study demonstrates that the presence of proliferating trypanosomes does not per se interfere with the neural activityof CNS tissues. Secondly, it provides, to the best of our knowledge, the first in vitro demonstration of intracellular forms of African trypanosomes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:08:47