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Titolo:
Optimisation of flow cytometric measurement of parasitaemia in plasmodium-infected mice
Autore:
Barkan, D; Ginsburg, H; Golenser, J;
Indirizzi:
Hebrew Univ Jerusalem, Kuvin Ctr Study Infect & Trop Dis, Dept Parasitol, Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel ept Parasitol, Jerusalem, Israel Hebrew Univ Jerusalem, Inst Life Sci, Dept Biol Chem, IL-91904 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91904 -91904 Jerusalem, Israel
Titolo Testata:
INTERNATIONAL JOURNAL FOR PARASITOLOGY
fascicolo: 5, volume: 30, anno: 2000,
pagine: 649 - 653
SICI:
0020-7519(20000424)30:5<649:OOFCMO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLS; FALCIPARUM; DYE;
Keywords:
flow cytometry; malaria; parasitaemia; Plasmodium berghei; Plasmodium vinckei petteri;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
8
Recensione:
Indirizzi per estratti:
Indirizzo: Golenser, J Hebrew Univ Jerusalem, Kuvin Ctr Study Infect & Trop Dis, DeptParasitol, Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel l, Jerusalem, Israel
Citazione:
D. Barkan et al., "Optimisation of flow cytometric measurement of parasitaemia in plasmodium-infected mice", INT J PARAS, 30(5), 2000, pp. 649-653

Abstract

Mouse malaria is often used as a model for drug testing. The results of drug trials are monitored by tedious (and consequently, sometimes inaccurate)microscopic counting of blood smears, or by flow cytometry. We suggest an improved, accurate and time-saving flow cytometric method for determinationof parasitaemias in mice infected with Plasmodium vinckei petteri or Plasmodium berghei. The method involves collection of drops of blood from the tail vein, fixation, storage, permeabilisation. staining and analysis with a visible range flow cytometer. Three nucleic acid dyes, YOYO-1, propidium iodide and acridine orange were compared. YOYO-1 was found to be the best stain for the discrimination of parasitised erythrocytes from non-infected ones. A good direct correlation was obtained between parasitaemia determined by conventional microscopy and parasitaemia measured by flow cytometry. Drugeffects could be assessed by the cytometric method. For the detection of low level of parasitemia. parasitised cells were treated with RNAse to completely cancel RNA-derived signals originating from host reticulocytes. This procedure also revealed discrete peaks arising from red cells infected withmultiple parasites or from parasites with different numbers of nuclei. (C)2000 Published by Elsevier Science Ltd on behalf of the Australian Societyfor Parasitology Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:46:10