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Titolo:
Detection of cysteine-proteinases in Leishmania amazonensis promastigotes using a cross-reactive antiserum
Autore:
Alves, CR; Corte-Real, S; Rosa, MDF; Giovanni-De-Simone, S;
Indirizzi:
FIOCRUZ, Inst Oswaldo Cruz, Dept Bioquim & Biol Mol, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ Rio De Janeiro Brazil BR-21045900 BC45900 Rio De Janeiro, Brazil FIOCRUZ, Inst Oswaldo Cruz, Dept Ultraestrutura & Biol Celular, Rio De Janeiro, Brazil FIOCRUZ Rio De Janeiro Brazil ra & Biol Celular, Rio De Janeiro, Brazil Univ Fed Fluminense, Inst Biol, Dept Biol Celular & Mol, Niteroi, RJ, Brazil Univ Fed Fluminense Niteroi RJ Brazil Celular & Mol, Niteroi, RJ, Brazil
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 2, volume: 186, anno: 2000,
pagine: 263 - 267
SICI:
0378-1097(20000515)186:2<263:DOCILA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEXICANA-MEXICANA; CATHEPSIN-B; GENE; AMASTIGOTES; PROTEASES; FORMS;
Keywords:
Leishmania amazonensis; cysteine-proteinase; anti-papain serum; immunocytochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Alves, CR FIOCRUZ, Inst Oswaldo Cruz, Dept Bioquim & Biol Mol, CP 926, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ CP 926 Rio De Janeiro Brazil BR-21045900 BCeiro, Brazil
Citazione:
C.R. Alves et al., "Detection of cysteine-proteinases in Leishmania amazonensis promastigotes using a cross-reactive antiserum", FEMS MICROB, 186(2), 2000, pp. 263-267

Abstract

Rabbit serum against the cysteine-proteinases papain has been employed forthe cellular localization of cysteine-proteinases of in Leishmania amazonensis promastigotes. By immunocytochemistry, immune complexes were found in the plasma membrane and in the flagella pocket of the parasite. The antiserum immunoprecipitated major iodinated proteins with molecular masses of 66,45, 28 and 24 kDa and a wide partitioning of the Triton X-114 detergent phase. The presence of cysteine-proteinase at the cell surface membrane was also suggested by the detection of proteolytic activity in living cells (19.0 mu g azocasein min(-1) 10(-5) promastigotes (1.0 S.D.)). (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:47:29