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Titolo:
Free radical production correlates with cell death in an in vitro model ofepilepsy
Autore:
Frantseva, MV; Velazquez, JLP; Hwang, PA; Carlen, PL;
Indirizzi:
Toronto Hosp, Res Inst, Playfair Neurosci Unit,Dept Med Neurol, Bloorview Epilepsy Programme, Toronto, ON M5T 2S8, Canada Toronto Hosp Toronto ON Canada M5T 2S8 ramme, Toronto, ON M5T 2S8, Canada
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 4, volume: 12, anno: 2000,
pagine: 1431 - 1439
SICI:
0953-816X(200004)12:4<1431:FRPCWC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR CALCIUM ACCUMULATION; ORGANOTYPIC HIPPOCAMPAL CULTURES; RAT HIPPOCAMPUS; EPILEPTIFORM ACTIVITY; SUPEROXIDE PRODUCTION; VITAMIN-E; IN-VITRO; OXYGEN; NEURONS; BRAIN;
Keywords:
bicuculline; glutathione; neurons; organotypic slices; vitamin E;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Carlen, PL Toronto Western Hosp, Playfair Neurosci Unit, McL 12-413,399 Bathurst St, Toronto, ON M5T 2S8, Canada Toronto Western Hosp McL 12-413,399 Bathurst St Toronto ON Canada M5T 2S8
Citazione:
M.V. Frantseva et al., "Free radical production correlates with cell death in an in vitro model ofepilepsy", EUR J NEURO, 12(4), 2000, pp. 1431-1439

Abstract

Free radical (FR) production, a major step in calcium-dependent neurodegeneration, has been linked to the generation of epileptiform activity and seizure-induced cell death. However, direct evidence of FR production in neurons during seizures has never been presented. Using hippocampal cultured slices we demonstrate that FRs are produced in CA3 but not CA1 pyramidal neurons during the rhythmic synchronous activity induced by the GABA(A) receptorantagonist bicuculline. The production of FRs (measured as changes in the fluorescence emission of dihydrorhodamine 123) was correlated with an increase in the baseline levels of intracellular calcium ([Ca2+](i)) estimated by fluo-3 injected into individual neurons via a patch pipette. [Ca2+](i) increased during spike bursting and returned to baseline levels after the burst termination in CA1, but not in CA3, pyramidal neurons where 'interburst'calcium concentrations progressively increased. Measurement of cell death,performed with propidium iodide 48 h after a 30-min exposure to bicuculline, revealed most prominent degeneration of pyramidal neurons in the CA3 pyramidal layer. The FR scavengers vitamin E and glutathione significantly reduced the seizure-induced neurodegeneration without supressing spontaneous epileptiform activity. These observations indicate that FR overproduction isrelated to seizure-induced neuronal death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 21:19:46