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Titolo:
Genetic diagnosis of micrometastasis based on SCC antigen mRNA in cervicallymph nodes of head and neck cancer
Autore:
Hamakawa, H; Fukizumi, M; Bao, Y; Sumida, T; Onishi, A; Tanioka, H; Sato, H; Yumoto, E;
Indirizzi:
Ehime Univ, Sch Med, Dept Oral & Maxillofacial Surg, Shigenobu, Ehime 7910295, Japan Ehime Univ Shigenobu Ehime Japan 7910295 Shigenobu, Ehime 7910295, Japan Ehime Univ, Sch Med, Dept Otolaryngol, Matsuyama, Ehime 790, Japan Ehime Univ Matsuyama Ehime Japan 790 aryngol, Matsuyama, Ehime 790, Japan
Titolo Testata:
CLINICAL & EXPERIMENTAL METASTASIS
fascicolo: 7, volume: 17, anno: 1999,
pagine: 593 - 599
SICI:
0262-0898(1999)17:7<593:GDOMBO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SQUAMOUS-CELL CARCINOMA; POLYMERASE CHAIN-REACTION; KERATIN-19 MESSENGER-RNA; UTERINE CERVIX; GASTRIC-CANCER; BREAST-CANCER; TUMOR-CELLS; EXPRESSION; OCCULT; TISSUE;
Keywords:
head and neck; lymph node; RT-PCR; SCC antigen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Hamakawa, H Ehime Univ, Sch Med, Dept Oral & Maxillofacial Surg, Shigenobu, Ehime 7910295, Japan Ehime Univ Shigenobu Ehime Japan 7910295 Ehime 7910295, Japan
Citazione:
H. Hamakawa et al., "Genetic diagnosis of micrometastasis based on SCC antigen mRNA in cervicallymph nodes of head and neck cancer", CLIN EXP M, 17(7), 1999, pp. 593-599

Abstract

This study is designed to assess gene expression of squamous cell carcinoma antigen (SCCA) mRNA to detect micrometastases in cervical lymph nodes (LNs) of head and neck cancer. We examined the expression of SCCA mRNA in 12 primary tumors and 212 cervical LNs (101 LNs taken from 8 patients with tongue cancer, 71 from 7 patients with gingival cancer, 19 from 2 patients withlaryngeal cancer, 9 from 2 patients with pharyngeal cancer, 7 from 1 patient with cancer of the buccal mucosa, and 5 from 1 patient with cancer of floor of the mouth). Detectability of metastatic LNs by nested and single reverse transcriptase-polymerase chain reaction (RT-PCR) was compared with semiserial sections (hematoxylin-eosin staining and keratin immunostaining). All primary tumors expressed SCCA mRNA. Of 198 histologically metastasis-negative nodes, SCCA mRNA was detected in 37 (18.7%) by nested PCR. Eleven micrometastatic foci in 9 LNs (4.6%) were discovered by semiserial sectioning. This suggests that SCCA mRNA is a promising tumor marker for detecting themicrometastases in cervical LNs of head and neck cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:42:32