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Titolo:
Contamination of a high-cell-density continuous bioreactor
Autore:
Domingues, L; Lima, N; Teixeira, JA;
Indirizzi:
Univ Minho, Ctr Engn Biol IBQF, P-4710057 Braga, Portugal Univ Minho Braga Portugal P-4710057 Biol IBQF, P-4710057 Braga, Portugal
Titolo Testata:
BIOTECHNOLOGY AND BIOENGINEERING
fascicolo: 5, volume: 68, anno: 2000,
pagine: 584 - 587
SICI:
0006-3592(20000605)68:5<584:COAHCB>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; SELECTIVE FLOCCULATION; FERMENTATIONS; RECYCLE; LACTOSE;
Keywords:
airlift bioreactor; continuous operation; flocculent S. cerevisiae; bacterial contamination;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Teixeira, JA Univ Minho, Ctr Engn Biol IBQF, P-4710057 Braga, Portugal Univ Minho Braga Portugal P-4710057 4710057 Braga, Portugal
Citazione:
L. Domingues et al., "Contamination of a high-cell-density continuous bioreactor", BIOTECH BIO, 68(5), 2000, pp. 584-587

Abstract

Continuous fermentations were carried out with a recombinant flocculent Saccharomyces cerevisiae Strain in an airlift bioreactor. Once operating under steady state at a dilution rate of 0.45 h(-1), the bioreactor was contaminated with Escherichia coil cells. The faster growing E. coli strain was washed out of the bioreactor and the recombinant, slower growing flocculatingS. cerevisiae strain remained as the only species detected in the bioreactor. Flocculation, besides allowing for the realization of high-cell-densitysystems with corresponding unusual high productivity, may be used as a selective property for controlling some contamination problems associated withprolonged continuous operation. (C) 2000 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 20:53:10