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Titolo:
Paper is a compatible bed for rat hepatocytes
Autore:
Mizuguchi, T; Mitaka, T; Sato, F; Mochizuki, Y; Hirata, K;
Indirizzi:
Sapporo Med Univ, Sch Med, Canc Res Inst, Dept Pathol,Chuo Ku, Sapporo, Hokkaido 0608556, Japan Sapporo Med Univ Sapporo Hokkaido Japan 0608556 , Hokkaido 0608556, Japan Sapporo Med Univ, Sch Med, Canc Res Inst, Dept Surg,Chuo Ku, Sapporo, Hokkaido 0608556, Japan Sapporo Med Univ Sapporo Hokkaido Japan 0608556 , Hokkaido 0608556, Japan
Titolo Testata:
ARTIFICIAL ORGANS
fascicolo: 4, volume: 24, anno: 2000,
pagine: 271 - 277
SICI:
0160-564X(200004)24:4<271:PIACBF>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYBRID ARTIFICIAL LIVER; BIOARTIFICIAL LIVER; CELL-SHAPE; CULTURE; POLYURETHANE; MAINTENANCE; EXPRESSION; MATRIX; DMSO; EGF;
Keywords:
rat hepatocytes; bioartificial liver;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Mitaka, T Sapporo Med Univ, Sch Med, Canc Res Inst, Dept Pathol,Chuo Ku, S-1,W-17, Sapporo, Hokkaido 0608556, Japan Sapporo Med Univ S-1,W-17 SapporoHokkaido Japan 0608556 , Japan
Citazione:
T. Mizuguchi et al., "Paper is a compatible bed for rat hepatocytes", ARTIF ORGAN, 24(4), 2000, pp. 271-277

Abstract

To develop an effective hybrid bioartificial liver (BAL) device, the material of the scaffold is very important to support hepatocytes that have bothgrowth ability and hepatic differentiated functions. In this study we usedpaper (Kimwipe, Kimberly-Clark Corp., Roswell, GA, U.S.A.) as a scaffold. Primary hepatocytes isolated from a normal adult rat liver could proliferate on the paper. The secretion of albumin into culture medium by the cells on the paper increased with time in culture and, compared to the cells on dishes, the amount of 48 h albumin secretion at Day 10 was two times larger. Perpendicular sections of hepatocytes on the paper revealed that the cells fell into cavities made by intersecting fibers, piled up, and formed three to four layers. The piled-up cells changed their shape from flat to cuboidal and enlarged their cytoplasm, which was rich in organelles such as mitochondria and peroxisomes with a nucleoid. In addition, they formed bile canalicular structures between the cells. Their morphological appearance was similar to in vivo hepatocytes. Paper (Kimwipe) may be a good candidate as a scaffold to make BAL device.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:28:41