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Titolo:
Multimicrobial sensor using microstructured three-dimensional electrodes based on silicon technology
Autore:
Konig, A; Reul, T; Harmeling, C; Spener, F; Knoll, M; Zaborosch, C;
Indirizzi:
Inst Chemo & Biosensor, D-48149 Munster, Germany Inst Chemo & Biosensor Munster Germany D-48149 D-48149 Munster, Germany Univ Munster, Inst Biochem, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 t Biochem, D-48149 Munster, Germany
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 9, volume: 72, anno: 2000,
pagine: 2022 - 2028
SICI:
0003-2700(20000501)72:9<2022:MSUMTE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIOCHEMICAL OXYGEN-DEMAND; YEAST ARXULA-ADENINIVORANS; MICROBIAL SENSOR; BOD BIOSENSOR; BIODEGRADABLE SUBSTANCES; GLUCOSE SENSOR; WATER; EFFICACY; LS3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Zaborosch, C Inst Chemo & Biosensor, Mendelstr 7, D-48149 Munster, GermanyInst Chemo & Biosensor Mendelstr 7 Munster Germany D-48149 y
Citazione:
A. Konig et al., "Multimicrobial sensor using microstructured three-dimensional electrodes based on silicon technology", ANALYT CHEM, 72(9), 2000, pp. 2022-2028

Abstract

Two microbial strains with different substrate spectra were immobilized separately within a single biosensor chip featuring four individually addressable platinum electrodes. These were sputtered onto the inner surface of four isolated pyramidal cavities ("containments") micromachined on a silicon wafer. The biosensor chip was integrated into a now-through system to measure the oxygen consumption of the immobilized microorganisms in the presenceof assimilable analytes. As a model system, a yeast for the determination of biochemical oxygen demand (BOD) and a strain capable of degrading polycyclic aromatic hydrocarbons (PAH) were chosen. It was shown that the simple and mass-producible containment sensor exhibits good performance data: lower detection limit 0.1 mg/L naphthalene and 1 mg/L sensor-BOD; calibration range up to 30 mg/L; precision 3-6%; response time 2-3 min; service life up to 40 days; shelf life at 4 degrees C 6 months. The versatility of the multimicrobial sensor was demonstrated by measuring ordinary municipal wastewater samples as well as various aqueous samples contaminated with PAH. The concept of a multimicrobial sensor not only enlarges the substrate spectrum for sum parameters such as BOD but leads to additional information which allows for a more differentiated and immediate knowledge of sample composition. Using chemometrical data analysis, the multimicrobial sensor lays a foundation for developing an "electronic tongue".

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:19:00