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Titolo:
Citrate release by perfused rat hearts: a window on mitochondrial cataplerosis
Autore:
Vincent, G; Comte, B; Poirier, M; Des Rosiers, C;
Indirizzi:
Univ Montreal, Dept Biochem, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 em, Montreal, PQ H3C 3J7, Canada Univ Montreal, Dept Nutr, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 tr, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 5, volume: 278, anno: 2000,
pagine: E846 - E856
SICI:
0193-1849(200005)278:5<E846:CRBPRH>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
FATTY-ACID OXIDATION; CARNITINE PALMITOYLTRANSFERASE-I; CORONARY-ARTERY DISEASE; ACETYL-COA CARBOXYLASE; MALONYL-COA; METABOLISM; PYRUVATE; CYCLE; OXALOACETATE; GLYCOLYSIS;
Keywords:
energy metabolism; citric acid cycle; C-13 mass isotopomer analysis; mitochondrial tricarboxylic acid transporter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Des Rosiers, C Ctr Hosp Univ Montreal, Hop Notre Dame, Lab Metab Intermediaire, Y-3616,1560 Sherbrooke St E, Montreal, PQ H2L 4M1, Canada Ctr Hosp Univ Montreal Y-3616,1560 Sherbrooke St E Montreal PQ Canada H2L 4M1
Citazione:
G. Vincent et al., "Citrate release by perfused rat hearts: a window on mitochondrial cataplerosis", AM J P-ENDO, 278(5), 2000, pp. E846-E856

Abstract

Cytosolic citrate is proposed to play a crucial role in substrate fuel selection in the heart. However, little is known about factors regulating the transfer of citrate from the mitochondria, where it is synthesized, to the cytosol. Further to our observation that rat hearts perfused under normoxiarelease citrate whose C-13 labeling pattern reflects that of mitochondrialcitrate (B. Comte, G. Vincent, B. Bouchard, and C. Des Rosiers. J. Biol. Chem. 272: 26117-26124, 1997), we report here data indicating that this citrate release is a specific process reflecting the mitochondrial efflux of citrate, a process referred to as cataplerosis. Indeed, measured rates of citrate release, which vary between 2 and 21 nmol/min, are modulated by the nature and concentration of exogenous substrates feeding acetyl-CoA (fatty acid) and oxaloacetate (lactate plus pyruvate) for the mitochondrial citrate synthase reaction. Such release rates that represent at most 2% of the citric acid cycle flux are in agreement with the activity of the mitochondrial tricarboxylate transporter whose participation is also substantiated by I) parallel variations in citrate release rates and tissue levels of citrate plus malate, the antiporter, and 2) a lowering of the citrate release rate by 1,2,3-benzenetricarboxylic acid, a specific inhibitor of the transporter. Taken together, the results from the present study indicate that citrate cataplerosis is modulated by substrate supply, in agreement with the role ofcytosolic citrate in fuel partitioning, and occurs, at least in part, through the mitochondrial tricarboxylate transporter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 03:57:22