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Titolo:
Cadmium induces caspase-mediated cell death: suppression by Bcl-2
Autore:
Kim, MS; Kim, BJ; Woo, HN; Kim, KW; Kim, KB; Kim, IK; Jung, YK;
Indirizzi:
Kwangju Inst Sci & Technol, Dept Life Sci, Puk Gu, Kwangju 500712, South Korea Kwangju Inst Sci & Technol Kwangju South Korea 500712 00712, South Korea
Titolo Testata:
TOXICOLOGY
fascicolo: 1, volume: 145, anno: 2000,
pagine: 27 - 37
SICI:
0300-483X(20000407)145:1<27:CICCDS>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ICE/CED-3 PROTEASE; INTERLEUKIN-1-BETA-CONVERTING ENZYME; THYMOCYTE APOPTOSIS; CYTOCHROME-C; ICE-LIKE; IN-VIVO; ELEGANS; FAMILY; DISEASE; ENCODES;
Keywords:
apoptosis; caspase; Bcl-2; CrmA; cadmium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Jung, YK Kwangju Inst Sci & Technol, Dept Life Sci, Puk Gu, 1 Oryong Dong,Kwangju 500712, South Korea Kwangju Inst Sci & Technol 1 Oryong Dong Kwangju South Korea 500712
Citazione:
M.S. Kim et al., "Cadmium induces caspase-mediated cell death: suppression by Bcl-2", TOXICOLOGY, 145(1), 2000, pp. 27-37

Abstract

Apoptosis is a process of active cell death and is characterized by activation of caspases, DNA fragmentation, and biochemical and morphological changes. To better understand apoptosis, we have characterized the dose- and time-dependent toxic effects of cadmium in Rat-1 fibroblasts. Staining of cells with phosphatidylserine (PS)-annexin V. Hoechst 33258 or Rhodamine 123 and Tunel assays showed that incubating cells with 10 mu M cadmium induced aform of cell death exhibiting typical characteristics of apoptosis, including cell shrinkage; externalization of PS, loss of mitochondria membrane potential, nuclear condensation and DNA fragmentation. Expression of Bcl-2 orCrmA each suppressed cadmium-induced cell death although Bcl-2 was somewhat more effective than CrmA. In vitro assay of caspase activity carried out using poly(ADP-ribose) polymerase (PARP) as a substrate as well as intracellular caspase assays using a fluorigenic caspase-3 substrate confirmed thatcaspase-3 is activated in Rat-1 cells undergoing cadmium-induced apoptosis. Both Asp-Glu-Val-Asp-aldehyde (DEVD-cho) and Tyr-Val-Ala -Asp-chloromethylketone (YVAD-cmk), selective inhibitors of caspase-3 and caspase-1, respectively, suppressed significantly cadmium-induced cell death. However, the nonselective caspase inhibitor, z-Val-Ala-Asp-floromethylketone (zVAD-fmk), was the most efficacious agent, almost completely blocking cadmium-induced cell death. Taken together, these results demonstrate that as in other forms of apoptosis, caspases play a central role in cadmium-induced cell death. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 13:47:06