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Titolo:
Successful implantation of in vitro-matured, electro-activated oocytes in the pig
Autore:
Kure-Bayashi, S; Miyake, M; Okada, K; Kato, S;
Indirizzi:
Kobe Univ, Grad Sch Sci & Technol, Kobe, Hyogo 6578501, Japan Kobe Univ Kobe Hyogo Japan 6578501 & Technol, Kobe, Hyogo 6578501, Japan Kobe Univ, Fac Agr, Dept Anim Sci, Kobe, Hyogo 6578501, Japan Kobe Univ Kobe Hyogo Japan 6578501 t Anim Sci, Kobe, Hyogo 6578501, Japan
Titolo Testata:
THERIOGENOLOGY
fascicolo: 5, volume: 53, anno: 2000,
pagine: 1105 - 1119
SICI:
0093-691X(20000315)53:5<1105:SIOIVE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR TRANSPLANTATION; IN-VITRO; PARTHENOGENETIC DEVELOPMENT; MOUSE OOCYTES; EMBRYOS; BLASTOCYST; INVITRO; GENOMES; STAGE; EGGS;
Keywords:
pig oocytes; parthenogenetic fetuses; implantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Miyake, M Kobe Univ, Grad Sch Sci & Technol, Kobe, Hyogo 6578501, Japan Kobe Univ Kobe Hyogo Japan 6578501 , Kobe, Hyogo 6578501, Japan
Citazione:
S. Kure-Bayashi et al., "Successful implantation of in vitro-matured, electro-activated oocytes in the pig", THERIOGENOL, 53(5), 2000, pp. 1105-1119

Abstract

In the present study, we derived parthenogenetic porcine fetuses from in vitro-matured oocytes following a simple activation process in order to evaluate their developmental limitations in-vivo. Follicular oocytes collected from gilts at local slaughterhouses were matured for 48 h. They were subjected to a single square pulse of direct current for 100 mu sec at 1,500 V/cmand then treated with 5 mu g/mL cytochalasin B for 4 h to obtain activateddiploid oocytes. The diploids were cultured in modified Whitten's medium until transfer. Diploids which had cleaved to the 2- and 3- to 4-cell stageswere transferred to oviducts of recipients. Live and/or dead parthenogenetic fetuses were recovered in 6 of 8 trials at 17, 18, 19, 24 and 29 d post activation. The total proportion of fetuses to transferred diploids was 31.3% (62/198). When fetuses were recovered at 19 d post activation, the proportion of development into fetuses was 71% (15/21). Our results, however, suggest that periods of gestation longer than 19 d resulted in a decrease of these proportions to 45% (18/40) at 24 d and to 18% (7/40) at 29 d. The hearts were beating in nearly all of the fetuses recovered at 19, 24 and 29 d post activation. Thus, parthenogenetic porcine diploids developed to at least the stage of limb-bud formation beyond the early heart-beating stage. Abnormalities were also externally visible on some fetuses. Formation of cyst-like structures in the heart and liver, and insufficient development of the head region and acephali were observed in some cases. (C) 2000 by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 04:49:30