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Titolo:
The Dewar photoproduct of thymidylyl(3 '-> 5 ')-thymidine (Dewar product) exhibits mutagenic behavior in accordance with the "A rule"
Autore:
Lee, JH; Bae, SH; Choi, BS;
Indirizzi:
Korea Adv Inst Sci & Technol, Dept Chem, Taejon 305701, South Korea Korea Adv Inst Sci & Technol Taejon South Korea 305701 5701, South Korea Korea Adv Inst Sci & Technol, Sch Mol Sci BK21, Taejon 305701, South KoreaKorea Adv Inst Sci & Technol Taejon South Korea 305701 5701, South Korea
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 9, volume: 97, anno: 2000,
pagine: 4591 - 4596
SICI:
0027-8424(20000425)97:9<4591:TDPOT'>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
RELAXATION MATRIX REFINEMENT; THYMINE CYCLOBUTANE DIMER; DNA DECAMER DUPLEX; ESCHERICHIA-COLI; SKIN-CANCER; CIS-SYN; DEOXYRIBONUCLEIC-ACID; APYRIMIDINIC SITES; 6-4 PHOTOPRODUCT; ABASIC SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Choi, BS Korea Adv Inst Sci & Technol, Dept Chem, 373-1 Kusong Dong, Taejon 305701,South Korea Korea Adv Inst Sci & Technol 373-1 Kusong Dong TaejonSouth Korea 305701
Citazione:
J.H. Lee et al., "The Dewar photoproduct of thymidylyl(3 '-> 5 ')-thymidine (Dewar product) exhibits mutagenic behavior in accordance with the "A rule"", P NAS US, 97(9), 2000, pp. 4591-4596

Abstract

In contrast to the highly mutagenic pyrimidine(6-4)pyrimidone photoproduct, its Dewar valence isomer (Dewar product) has low mutagenic potential and produces a broad range of mutations [LeClerc, J. E., Borden. A. & Lawrence,C. W. (1991) Proc. Natl. Acad. Sci. USA 88. 9685-9689]. To determine the origin of the mutagenic property of the Dewar product, we used experimental NMR restraints and molecular dynamics to determine the solution structure of a Dewar-lesion DNA decamer duplex. This DNA decamer duplex (DW/GA duplex)contains a mismatched base pair between the 3' T residue of the Dewar lesion (T6) and an opposed G residue (G15). The 3' T (T6) of the Dewar lesion formed stable hydrogen bonds with the opposing G15 residue. However, the helical bending and unwinding angles of the DW/GA duplex were much larger thanthose of a second duplex that contains the Dewar lesion and opposing A15 and A16 residues (DW/AA duplex). The DW/GA duplex showed poorer stacking interactions at the two bases of the Dewar product and at the adjacent A7.T14 base pair than did the DW/AA duplex. These structural features imply that no thermal stability or conformational benefit is obtained by incorporating a G instead of an A opposite the 3' T of the Dewar lesion. These propertiesmay thus facilitate the preferential incorporation of an A in accordance with the A rule during translesion replication and lead to the low frequencyof 3' T-->C mutations observed at this site.

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Documento generato il 02/04/20 alle ore 05:57:03