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Titolo:
Evidence of protein synthesis during resuscitation of ischemically damagedcanine pancreas by the two-layer method
Autore:
Matsumoto, S; Fujino, Y; Suzuki, Y; Tanioka, Y; Muramatsu, S; Sugimoto, T; Ku, YS; Yasunami, Y; Kuroda, Y;
Indirizzi:
Kobe Univ, Sch Med, Dept Surg 1, Chuo Ku, Kobe, Hyogo 6500017, Japan Kobe Univ Kobe Hyogo Japan 6500017 1, Chuo Ku, Kobe, Hyogo 6500017, Japan Fukuoka Univ, Sch Med, Dept Surg 1, Fukuoka 81401, Japan Fukuoka Univ Fukuoka Japan 81401 Med, Dept Surg 1, Fukuoka 81401, Japan
Titolo Testata:
PANCREAS
fascicolo: 4, volume: 20, anno: 2000,
pagine: 411 - 414
SICI:
0885-3177(200005)20:4<411:EOPSDR>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLD-STORAGE METHOD; 2-LAYER METHOD; PRESERVATION; OXYGENATION; GRAFTS;
Keywords:
protein synthesis; two-layer method; warm ischemia; pancreas transplantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Fujino, Y Kobe Univ, Sch Med, Dept Surg 1, Chuo Ku, 7-5-2 Kusunoki-cho, Kobe, Hyogo 6500017, Japan Kobe Univ 7-5-2 Kusunoki-cho Kobe Hyogo Japan 6500017 017, Japan
Citazione:
S. Matsumoto et al., "Evidence of protein synthesis during resuscitation of ischemically damagedcanine pancreas by the two-layer method", PANCREAS, 20(4), 2000, pp. 411-414

Abstract

The two-layer method, which supplies sufficient oxygen to the canine pancreas graft and allows adenotriphosphate synthesis within the graft, resuscitates the ischemically damaged pancreas graft during mild hypothermic (20 degrees C) preservation, but the mechanisms are unknown. The purpose of this study was to determine whether protein synthesis was performed on ischemically damaged pancreas graft during preservation by the two-layer method in acanine autotransplantation model. The pancreas,grafts subjected to 90 minutes of warm ischemia were preserved by the two-layer method (perfluorochemical/University of Wisconsin solution) at 20 degrees C for 5 hours. Graft viability was judged from graft survival after autotransplantation. DNA, RNA,and protein synthesis was quantitated by determining incorporation of tritiated thymidine, tritiated uridine, and tritiated leucine, respectively, during preservation. Significant increases in RNA and subsequent protein syntheses were observed during preservation by the two-layer method. In contrast, DNA synthesis did not follow RNA and protein synthesis. We conclude thatprotein is synthesized in the process of post ischemic cellular recovery of the pancreas graft during mild hypothermic preservation by the two-layer method.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:07:07