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Titolo:
Insulin stimulates the release of a subset of GPI-anchored proteins in a G-protein independent manner
Autore:
Movahedi, S; Pang, S; Hooper, NM;
Indirizzi:
Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England
Titolo Testata:
MOLECULAR MEMBRANE BIOLOGY
fascicolo: 1, volume: 17, anno: 2000,
pagine: 41 - 45
SICI:
0968-7688(200001/03)17:1<41:ISTROA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE; GTP-BINDING PROTEINS; GLYCOSYL-PHOSPHATIDYLINOSITOL; SYNTHETIC PHOSPHOINOSITOLGLYCANS; MEMBRANE DIPEPTIDASE; RAT ADIPOCYTES; CELLS; INVOLVEMENT; MEDIATORS; TOXIN;
Keywords:
GPI anchors; phospholipase C; G proteins; 3T3 adipocytes; pertussis toxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Hooper, NM Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire,England Univ Leeds Leeds W Yorkshire England LS2 9JT orkshire, England
Citazione:
S. Movahedi et al., "Insulin stimulates the release of a subset of GPI-anchored proteins in a G-protein independent manner", MOL MEMBR B, 17(1), 2000, pp. 41-45

Abstract

The glycosyl-phosphatidylinositol anchored protein, membrane dipeptidase (EC 3.4.13.19) is released from the surface of 3T3-L1 adipocytes in responseto insulin treatment through the action of a phospholipase C. The present study investigates the role of guanine-nucleotide binding proteins (G-proteins) in this process. Treatment of permeabilized 3T3-L1 adipocytes with GTPgamma S did not cause release of membrane dipeptidase into the medium, while GDP beta S did not inhibit the insulin-stimulated release of membrane dipeptidase. Other activators of G-proteins, including the tetradecapeptide mastoparan, pertussis toxin and AIF(3), also caused no significant release of membrane dipeptidase from the surface of the 3T3-L1 adipocytes. From these observations it is concluded that G-proteins are not involved in the insulin-stimulated release of membrane dipeptidase. Although X-Pro aminopeptidase (EC 3.4.11.9) is GPI-anchored in 3T3-L1 adipocytes as shown by digestionwith bacterial phosphatidylinositol-specific phospholipase C, it was not released upon insulin treatment of the cells, indicating that only a subset of the GPI-anchored proteins are susceptible to insulin-stimulated release.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 19:39:49