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Titolo:
Optical properties of fluorochromes promising for use in biological microchips
Autore:
Kotova, EY; Kreindlin, EY; Barsky, VE; Mirzabekov, AD;
Indirizzi:
Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 117984, Russia Russian Acad Sci Moscow Russia 117984 l Biol Inst, Moscow 117984, Russia Biochip Technol Ctr, Argonne, IL 60439 USA Biochip Technol Ctr Argonne ILUSA 60439 chnol Ctr, Argonne, IL 60439 USA
Titolo Testata:
MOLECULAR BIOLOGY
fascicolo: 2, volume: 34, anno: 2000,
pagine: 266 - 271
SICI:
0026-8933(200003/04)34:2<266:OPOFPF>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA; HYBRIDIZATION;
Keywords:
fluorescent labels; fluorescently labeled oligonucleotides; spectral analysis; hybridization; biological microchips;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Kotova, EY Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 117984, Russia Russian Acad Sci Moscow Russia 117984 , Moscow 117984, Russia
Citazione:
E.Y. Kotova et al., "Optical properties of fluorochromes promising for use in biological microchips", MOL BIOL, 34(2), 2000, pp. 266-271

Abstract

Fourteen fluorochromes (free and oligonucleotide-bound) and five ligands commonly used to quantitatively assess DNA duplexes or complexes with proteins in microchips were studied by measuring their fluorescence excitation and emission spectra. The spectral changes are described that were caused by oligonucleotide binding, solution hybridization, or varying the temperature. The data are discussed from the standpoint of applicability and limitations of the fluorochromes and the ligands in qualitative and quantitative assays for DNA duplexes in microchips.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 08:33:35