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Titolo:
Effects of various fluorochromes and competition between labeled oligonucleotides on their hybridization to oligonucleotides immobilized on biological microchips
Autore:
Kotova, EY; Kreindlin, EY; Barsky, VE; Mirzabekov, AD;
Indirizzi:
Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 117989, Russia Russian Acad Sci Moscow Russia 117989 l Biol Inst, Moscow 117989, Russia Biochip Technol Ctr, Argonne, IL 60439 USA Biochip Technol Ctr Argonne ILUSA 60439 chnol Ctr, Argonne, IL 60439 USA
Titolo Testata:
MOLECULAR BIOLOGY
fascicolo: 2, volume: 34, anno: 2000,
pagine: 207 - 214
SICI:
0026-8933(200003/04)34:2<207:EOVFAC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA;
Keywords:
biological microchips; hybridization; hybridization efficiency; fluorescently labeled oligonucleotides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Kotova, EY Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 117989, Russia Russian Acad Sci Moscow Russia 117989 , Moscow 117989, Russia
Citazione:
E.Y. Kotova et al., "Effects of various fluorochromes and competition between labeled oligonucleotides on their hybridization to oligonucleotides immobilized on biological microchips", MOL BIOL, 34(2), 2000, pp. 207-214

Abstract

Some technical tips are described on how to improve the discrimination between perfect and imperfect duplexes formed by hybridization of fluorescently labeled oligonucleotides to biological microchips. Model experiments wereperformed to assess the precision of the method. Effects of labeling on the efficiency of hybridization and some properties of competitive hybridization were studied using short synthetic oligonucleotides and three most popular fluorochromes as examples.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:13:22