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Titolo:
Procedures to characterize and study P-2Z/P2X(7) purinoceptor: Flow cytometry as a promising practical, reliable tool
Autore:
Nihei, OK; Savino, W; Alves, LA;
Indirizzi:
Inst Oswaldo Cruz, Dept Imunol, Lab Pesquisas Timo, BR-21045900 Rio De Janeiro, Brazil Inst Oswaldo Cruz Rio De Janeiro Brazil BR-21045900 BCDe Janeiro, Brazil
Titolo Testata:
MEMORIAS DO INSTITUTO OSWALDO CRUZ
fascicolo: 3, volume: 95, anno: 2000,
pagine: 415 - 428
SICI:
0074-0276(200005/06)95:3<415:PTCASP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
P(2Z) PURINERGIC RECEPTORS; PAROTID ACINAR-CELLS; GATED ION CHANNELS; NF-KAPPA-B; EXTRACELLULAR ATP; P2X(7) RECEPTOR; HUMAN-LYMPHOCYTES; PLASMA-MEMBRANE; PHOSPHOLIPASE-D; NUCLEOTIDE RECEPTOR;
Keywords:
flow cytometry; purinergic receptors; P2X(7) receptor; ethidium bromide; extracellular ATP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
120
Recensione:
Indirizzi per estratti:
Indirizzo: Alves, LA Inst Oswaldo Cruz, Dept Imunol, Lab Pesquisas Timo, Av Brasil 4365, BR-21045900 Rio De Janeiro, Brazil Inst Oswaldo Cruz Av Brasil 4365 RioDe Janeiro Brazil BR-21045900 BC
Citazione:
O.K. Nihei et al., "Procedures to characterize and study P-2Z/P2X(7) purinoceptor: Flow cytometry as a promising practical, reliable tool", MEM I OSW C, 95(3), 2000, pp. 415-428

Abstract

The expression of P-2Z/P2X(7) purinoceptor in different cell types is wellestablished. This receptor is a member of the ionotropic P2X receptor family, which is composed by seven cloned receptor subtypes (P2X(1) - P2X(7)). Interestingly, the P-2Z/P2X(7) has a unique feature of being linked to a non-selective pore which allows the passage of molecules up to 900 Da depending on the cell type. Early studies of P-2Z/P2X(7) purinoceptor were exclusively based on classical pharmacological studies but the recent tools of molecular biology have enriched;he analysis of the receptor expression. The majority of assays and techniques chosen so far to study the expression of P-2Z/P2X(7) receptor explore directly or indirectly the effects of the opening of P-2Z/P2X(7) linked pore. In this review we describe the main techniques used to study, the expression and functionality of P-2Z/P2X(7) receptor Additionally, the increasing need and importance of a multifunctional analysis of P-2Z/P2X(7) expression based on flow cytometry technology is discussed, as well as the adoption of a more complete analysis of P-2Z/P2X(7) expression involving different techniques.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:23:21