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Titolo:
Human Kaposi's sarcoma cell-mediated tumorigenesis in human immunodeficiency type 1 Tat-expressing transgenic mice
Autore:
Prakash, O; Tang, ZY; He, YE; Ali, MS; Coleman, R; Gill, J; Farr, G; Samaniego, F;
Indirizzi:
Alton Ochsner Med Fdn & Ochsner Clin, Mol Oncol Lab, New Orleans, LA 70121USA Alton Ochsner Med Fdn & Ochsner Clin New Orleans LA USA 70121 LA 70121USA Alton Ochsner Med Fdn & Ochsner Clin, Dept Pathol, New Orleans, LA 70121 USA Alton Ochsner Med Fdn & Ochsner Clin New Orleans LA USA 70121 A 70121 USA Univ Maryland, Inst Human Virol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Human Virol, Baltimore, MD 21201 USA Univ Maryland, Greenbaum Canc Ctr, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 um Canc Ctr, Baltimore, MD 21201 USA
Titolo Testata:
JOURNAL OF THE NATIONAL CANCER INSTITUTE
fascicolo: 9, volume: 92, anno: 2000,
pagine: 721 - 728
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; FIBROBLAST GROWTH-FACTOR; NECROSIS-FACTOR-ALPHA; VIRUS TYPE-1; ENDOTHELIAL-CELLS; HIV-1 INFECTION; INFLAMMATORY CYTOKINES; FLK-1/KDR RECEPTOR; IV COLLAGENASE; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Prakash, O Alton Ochsner Med Fdn & Ochsner Clin, Mol Oncol Lab, 1516 Jefferson Highway, New Orleans, LA 70121 USA Alton Ochsner Med Fdn & Ochsner Clin 1516 Jefferson Highway New Orleans LA USA 70121
Citazione:
O. Prakash et al., "Human Kaposi's sarcoma cell-mediated tumorigenesis in human immunodeficiency type 1 Tat-expressing transgenic mice", J NAT CANC, 92(9), 2000, pp. 721-728

Abstract

Background: The human immunodeficiency virus type 1 (HIV-1) transactivator(Tat) protein has been linked to the development and course of Kaposi's sarcoma (KS) associated with acquired immunodeficiency disease syndrome (AIDS-KS), Tat is an 86-101 amino-acid protein encoded by two exons, To evaluatethe growth-promoting effects of Tat in AIDS-KS in vivo, we developed transgenic mice expressing the one-exon-encoded 72 amino-acid protein (Tat(72)) and the two-exon-encoded 86 amino-acid protein (Tat(86)). Methods: Human KSSLK cells were injected subcutaneously into CD4(+) T-cell-depleted male mice, and the tumors that formed after 3-4 weeks were recovered and analyzed for the expression of Tat protein(s), different cytokine messenger RNAs (mRNAs), and matrix metalloproteinases (MMPs), All statistical tests were two-sided. Results: The average tumor weight was maximum in Tat(86) mice (similar to 600 mg) compared with Tat(72) (similar to 200 mg) and nontransgenic (similar to 100 mg) mice (P<.005). Histologic examination of tumors showed spindle-shaped SLK cells with prominent infiltrates of inflammatory cells. All of the tumors from Tat mice expressed abundant Tat mRNA, suggesting thatthe infiltrating mouse cells actively expressed Tat. A comparison of the growth-promoting cytokines in the tumors from Tat(86)-transgenic and nontransgenic mice showed that the expression of the following cytokines was substantially increased in the tumors of the Tat,, mice: tumor necrosis factor-alpha, interleukin 6, interleukin 8, granulocyte-macrophage colony-stimulating factor, and basic fibroblast growth factor. Furthermore, these tumors showed abundant expression of a 105-kd MMP activity associated with infiltrates of host leukocytes in the lesions. Conclusion: Our in vivo data clearly suggest that extracellular Tat can contribute to the growth and tumorigenesis of human KS cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:09:14