Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Temperature dependence of nitrate reductase activity in marine phytoplankton: Biochemical analysis and ecological, implications
Autore:
Gao, Y; Smith, GJ; Alberte, RS;
Indirizzi:
Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USA Univ So Calif LosAngeles CA USA 90089 iol Sci, Los Angeles, CA 90089 USA Moss Landing Marine Labs, Moss Landing, CA 95039 USA Moss Landing Marine Labs Moss Landing CA USA 95039 Landing, CA 95039 USA Univ Calif Los Angeles, Dept Biol, Los Angeles, CA 90024 USA Univ Calif Los Angeles Los Angeles CA USA 90024 Los Angeles, CA 90024 USA
Titolo Testata:
JOURNAL OF PHYCOLOGY
fascicolo: 2, volume: 36, anno: 2000,
pagine: 304 - 313
SICI:
0022-3646(200004)36:2<304:TDONRA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIATOM SKELETONEMA-COSTATUM; GLUTAMINE-SYNTHETASE; HIGHER-PLANTS; HIGH-NUTRIENT; LIGHT; ASSIMILATION; GROWTH; ADAPTATION; ASSAY; PHOTOSYNTHESIS;
Keywords:
chromophyte; chlorophyte; marine phytoplankton; nitrate assimilation; nitrate reductase; thermal stability; vascular plants;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Gao, Y Rutgers State Univ, Ctr Agr & Mol Biol, Foran Hall,Dudley Rd, New Brunswick, NJ 08901 USA Rutgers State Univ Foran Hall,Dudley Rd New Brunswick NJ USA 08901
Citazione:
Y. Gao et al., "Temperature dependence of nitrate reductase activity in marine phytoplankton: Biochemical analysis and ecological, implications", J PHYCOLOGY, 36(2), 2000, pp. 304-313

Abstract

The temperature dependence of NADH:NR activity was examined in several marine phytoplankton species and vascular plants. These species inhabit divergent thermal environments, including the chromophytes Skeletonema costatum (12-15 degrees C), Skeletonema tropicum (18-25 degrees C), Thalassiosira antarctica (-2 to 4 degrees C), and Phaeocystis antarctica (-2 to 4 degrees C), the green alga Dunaliella tertiolecta (14-28 degrees C), and the vascularplants Cucurbita maxima (20-35 degrees C) and Zea mays (20-25 degrees C), Despite the difference in growth habitats, similar temperature response curves were observed among the chromophytic phytoplankton, with temperatures optimal for NR activity being between 10-20 degrees C. In contrast, the chlorophyll b-containing alga and vascular plants exhibited optimal temperatures for NR activity above 30 degrees C. Such dramatic differences in NR thermal characteristics from the two taxonomic groups reflect a divergence in NRstructure that may be associated with the evolutionary diversification of chromophytes and chlorophytes. Further, it suggests a potential contribution of the thermal performance of NR to the geographic distributions, seasonal abundance patterns, and species composition pf phytoplankton communities. NR partial activities, which assess the individual functions of Mo-pterin and FAD domains, were evaluated on NR purified from S. costatum to determine the possible causes for high temperature (>20 degrees C) inactivation of NR from chromophytes. It was found that the FAD domain and electron transport among redox centers were sensitive to elevated temperatures. S, costatumcells grown at 5, 15, and 25 degrees C exhibited an identical optimal temperature (15 degrees C) for NADH:NR activity, whereas the maximal NR activity and NR protein levels differed and were positively correlated with growthtemperature and growth rate, These findings demonstrate that thermal acclimation of NO3- reduction capacity is largely at the level of NR protein expression. The consequences of these features on NO3- utilization are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 18:39:39