Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Genetically and epidemiologically related "non-syncytium-inducing" isolates of HIV-1 display heterogeneous growth patterns in macrophages
Autore:
Aquino-de Jesus, MJ; Anders, C; Miller, G; Sleasman, JW; Goodenow, MM; Andiman, WA;
Indirizzi:
Yale Univ, Sch Med, Dept Pediat, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 ed, Dept Pediat, New Haven, CT 06520 USA Yale Univ, Dept Epidemiol & Publ Hlth, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 iol & Publ Hlth, New Haven, CT 06520 USA Univ Florida, Coll Med, Dept Pathol Immunol & Lab Med, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 Lab Med, Gainesville, FL 32610 USA Univ Florida, Coll Med, Dept Pediat, Div Infect Dis & Immunol, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 Immunol, Gainesville, FL 32610 USA
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 2, volume: 61, anno: 2000,
pagine: 171 - 180
SICI:
0146-6615(200006)61:2<171:GAER"I>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; INFECTED CHILDREN; CELL-LINES; CHEMOKINE RECEPTORS; MONOCLONAL-ANTIBODY; TYPE-1 HIV-1; PHENOTYPE; TROPISM; CORECEPTOR; AIDS;
Keywords:
viral phenotype; viral isolates; pediatric HIV infection; monocyte-derived macrophages; non-syncytium-inducing; dual tropism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Andiman, WA Yale Univ, Sch Med, Dept Pediat, 333 Cedar St, New Haven, CT 06520 USA Yale Univ 333 Cedar St New Haven CT USA 06520 en, CT 06520 USA
Citazione:
M.J. Aquino-de Jesus et al., "Genetically and epidemiologically related "non-syncytium-inducing" isolates of HIV-1 display heterogeneous growth patterns in macrophages", J MED VIROL, 61(2), 2000, pp. 171-180

Abstract

The objective of this study was to identify phenotypic parameters that could distinguish among seemingly homogeneous non-syncytium-inducing (NSI) viruses and that might provide a surrogate marker for clinical progression in pediatric human immunodeficiency virus type 1 (HIV-1) infection. We undertook a pilot analysis of 15 independent HIV-1 isolates collected prospectively from two mothers and their four children who displayed a spectrum of disease stages ranging from CDC categories Al to C3. Viruses were evaluated fortheir ability to replicate in primary cells (including monocyte-derived macrophages [MDM]) and cell lines, for their co-receptor preference and for genetic features of the V3 hypervariable domain of env. Virtually all isolates displayed NSI phenotypes that were restricted in their capacity to replicate in cell lines and displayed V3 loops with uniformly low net positive charges. NSI viruses from two symptomatic children and one mother were macrophage-tropic, whereas NSI isolates from two asymptomatic children were unable to replicate in MDM and were designated primary lymphotropic viruses. Only one isolate was syncytium-inducing (SI), replicated in a variety of celllines and in MDM, used multiple co-receptors, and was dual tropic, rather than a mixture of T-cell tropic and M-tropic viruses, as assessed by genetic analysis. Phenotypic heterogeneity among NSI viruses is revealed in the ability of isolates to replicate in MDM. This characteristic is related to disease stage and provides a potentially new in vitro criterion to distinguish among NSI isolates that is unlinked to other surrogate markers. (C) 2000Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:44:49