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Titolo:
Chromatographic separation of chlorthalidone enantiomers using beta-cyclodextrins as chiral additives
Autore:
Herraez-Hernandez, R; Campins-Falco, P;
Indirizzi:
Univ Valencia, Dept Analyt Chem, E-46100 Valencia, Spain Univ Valencia Valencia Spain E-46100 nalyt Chem, E-46100 Valencia, Spain
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 740, anno: 2000,
pagine: 169 - 177
SICI:
1387-2273(20000414)740:2<169:CSOCEU>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; STATIONARY-PHASE; URINE; ELECTROPHORESIS; DIURETICS; HPLC;
Keywords:
enantiomer separation; chlorthalidone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Campins-Falco, P Univ Valencia, Dept Analyt Chem, Dr Moliner 50, E-46100 Valencia, Spain Univ Valencia Dr Moliner 50 Valencia Spain E-46100 Spain
Citazione:
R. Herraez-Hernandez e P. Campins-Falco, "Chromatographic separation of chlorthalidone enantiomers using beta-cyclodextrins as chiral additives", J CHROMAT B, 740(2), 2000, pp. 169-177

Abstract

Different beta-cyclodextrins have been tested as chiral additives in the mobile phase for the chromatographic analysis of chlorthalidone enantiomers in a C-18 LiChrospher (125x4 mm I.D.) column. The effect on enantioresolution of different parameters was studied: composition of the mobile phase (percentage of organic solvent, type of buffer and pH), mobile phase flow-rate, and type and concentration of beta-cyclodextrin. A 25:75 mixture of methanol and 0.1 M phosphate buffer, pH 4, containing 2% triethylamine (v/v), and 12.5 mM beta-cyclodextrin, at a flow-rate of 0.8 ml/min, was found to be the best option for the resolution of chlorthalidone enantiomers. Under such conditions, linear calibration curves were obtained in the 0.5-20-mu g/mlinterval using UV detection at 230 nm. The limit of detection for both isomers was 50 ng/ml. The utility of the described assay has been tested by analyzing chlorthalidone in different pharmaceutical preparations. Examples of application to biological samples are also given. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 11/07/20 alle ore 16:51:33