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Titolo:
Electromechanical coupling between skeletal and cardiac muscle: Implications for infarct repair
Autore:
Reinecke, H; MacDonald, GH; Hauschka, SD; Murry, CE;
Indirizzi:
Univ Washington, Dept Pathol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 , Dept Pathol, Seattle, WA 98195 USA Univ Washington, Dept Otolaryngol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 t Otolaryngol, Seattle, WA 98195 USA Univ Washington, Dept Biochem, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Dept Biochem, Seattle, WA 98195 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 3, volume: 149, anno: 2000,
pagine: 731 - 740
SICI:
0021-9525(20000501)149:3<731:ECBSAC>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
COCHLEAR NUCLEUS NEURONS; LONG-TERM SURVIVAL; N-CADHERIN; INTERCELLULAR COMMUNICATION; GAP-JUNCTIONS; MYOCARDIAL REGENERATION; MYOBLAST FUSION; BORDER ZONE; RAT; DIFFERENTIATION;
Keywords:
skeletal myocytes; cardiomyocytes; electromechanical coupling; N-cadherin; connexin43;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Murry, CE Univ Washington, Dept Pathol, Box 357470,Room E-520 HSB, Seattle, WA 98195USA Univ Washington Box 357470,Room E-520 HSB Seattle WA USA 98195 A
Citazione:
H. Reinecke et al., "Electromechanical coupling between skeletal and cardiac muscle: Implications for infarct repair", J CELL BIOL, 149(3), 2000, pp. 731-740

Abstract

Skeletal myoblasts form grafts of mature muscle in injured hearts, and these grafts contract when exogenously stimulated. It is not known, however, whether cardiac muscle can form electromechanical junctions with skeletal muscle and induce its synchronous contraction. Here, we report that undifferentiated rat skeletal myoblasts expressed N-cadherin and connexin43, major adhesion and gap junction proteins of the intercalated disk, yet both proteins were markedly downregulated after differentiation into myotubes. Similarly, differentiated skeletal muscle grafts in injured hearts had no detectable N-cadherin or connexin43; hence, electromechanical coupling did not occur after in vivo grafting. In contrast, when neonatal or adult cardiomyocytes were cocultured with skeletal muscle, similar to 10% of the skeletal myotubes contracted in synchrony with adjacent cardiomyocytes. Isoproterenol increased myotube contraction rates by 25% in coculture without affecting myotubes in monoculture, indicating the cardiomyocytes were the pacemakers. The gap junction inhibitor heptanol aborted myotube contractions but left spontaneous contractions of individual cardiomyocytes intact, suggesting myotubes were activated via gap junctions. Confocal microscopy revealed the expression of cadherin and connexin43 at junctions between myotubes and neonatal or adult cardiomyocytes in vitro. After microinjection, myotubes transferred dye to neonatal cardiomyocytes via gap junctions. Calcium imaging revealed synchronous calcium transients in cardiomyocytes and myotubes. Thus, cardiomyocytes can form electromechanical junctions with some skeletal myotubes in coculture and induce their synchronous contraction via gap junctions. Although the mechanism remains to be determined, if similar junctions could be induced in vivo, they might be sufficient to make skeletal muscle grafts beat synchronously with host myocardium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 23:26:06