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Titolo:
An NF-Y binding site is important for basal, but not gonadotropin-releasing hormone-stimulated, expression of the luteinizing hormone beta subunit gene
Autore:
Keri, RA; Bachmann, DJ; Behrooz, A; Herr, BD; Ameduri, RK; Quirk, CC; Nilson, JH;
Indirizzi:
Case Western Reserve Univ, Sch Med, Dept Pharmacol, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 17, volume: 275, anno: 2000,
pagine: 13082 - 13088
SICI:
0021-9258(20000428)275:17<13082:ANBSII>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEROIDOGENIC FACTOR-I; GROWTH-RESPONSE PROTEIN-1; ORPHAN NUCLEAR RECEPTOR; TRANSGENIC MICE; PROMOTER; TRANSCRIPTION; PITUITARY; ELEMENTS; SYNERGY; ACTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Nilson, JH Case Western Reserve Univ, Sch Med, Dept Pharmacol, 2109 Adelbert Rd, Cleveland, OH 44106 USA Case Western Reserve Univ 2109 Adelbert Rd Cleveland OH USA 44106
Citazione:
R.A. Keri et al., "An NF-Y binding site is important for basal, but not gonadotropin-releasing hormone-stimulated, expression of the luteinizing hormone beta subunit gene", J BIOL CHEM, 275(17), 2000, pp. 13082-13088

Abstract

Regulated synthesis of luteinizing hormone (LH) re quires coordinated transcriptional control of the alpha and LH beta subunits in pituitary gonadotropes. Several cis-acting elements and trans-acting factors have been defined for control of the LH beta promoter through heterologous cell culture models. In this report, we describe the identification of bipartite NF-Y (CBF/CP1) binding sites within the proximal bovine LH beta promoter. When multimerized, one of these sites activates the heterologous, minimal HSV thymidine kinase promoter in the gonadotrope-derived cell line alpha T3-1. The functional role of the promoter-distal site in regulating the full-length bovine LH beta promoter was assessed in vivo using transgenic mice harboring a mutant promoter linked to the chloramphenicol acetyltransferase reporter gene. While this element is important for conferring high level activity of the LH beta promoter in pituitary, it does not appear to be essential for mediating gonadotropin-releasing hormone (GnRH) regulation. This is the first characterization of a cia-acting element within this GnRH-dependent promoter that is restricted to regulating basal expression and not GnRH-induced activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 00:20:15