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Titolo:
The zinc finger repressor, ZBP-89, binds to the silencer element of the human vimentin gene and complexes with the transcriptional activator, Sp1
Autore:
Wieczorek, E; Lin, ZL; Perkins, EB; Law, DJ; Merchant, JL; Zehner, ZE;
Indirizzi:
Virginia Commonwealth Univ, Dept Biochem & Mol Biophys, Richmond, VA 23298USA Virginia Commonwealth Univ Richmond VA USA 23298 s, Richmond, VA 23298USA Virginia Commonwealth Univ, Massey Canc Ctr, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 nternal Med, Ann Arbor, MI 48109 USA Univ Michigan, Dept Physiol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 ept Physiol, Ann Arbor, MI 48109 USA Univ Michigan, Howard Hughes Med Inst, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 es Med Inst, Ann Arbor, MI 48109 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 17, volume: 275, anno: 2000,
pagine: 12879 - 12888
SICI:
0021-9258(20000428)275:17<12879:TZFRZB>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
KERATIN INTERMEDIATE FILAMENTS; TUMOR-SUPPRESSOR GENE; I TAX PROTEIN; NF-KAPPA-B; NEGATIVE ELEMENT; CELL-CYCLE; EXPERIMENTAL COEXPRESSION; ANTISILENCER ELEMENT; EPITHELIAL-CELLS; MAMMALIAN-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Zehner, ZE Virginia Commonwealth Univ, Dept Biochem & Mol Biophys, POB 980614,Med Coll Virginia Campus, Richmond, VA 23298 USA Virginia Commonwealth Univ POB 980614,Med Coll Virginia Campus Richmond VA USA 23298
Citazione:
E. Wieczorek et al., "The zinc finger repressor, ZBP-89, binds to the silencer element of the human vimentin gene and complexes with the transcriptional activator, Sp1", J BIOL CHEM, 275(17), 2000, pp. 12879-12888

Abstract

Vimentin is a component of the eukaryotic cytoskeleton belonging to the family of intermediate filament proteins. It exhibits a complex pattern of tissue- and development-specific expression. It is also a marker of the metastatic potential of many tumor cells. Previously, the human vimentin promoter was shown to contain several regions for the binding of positive and negative acting regulatory factors. Until now, the silencer element, which shuts down vimentin synthesis in selected tissues during development, was not precisely localized; nor was its binding protein known. In vivo DMS footprinting by ligation-mediated PCR delineated the position of guanine residues important to vimentin expression. Transient transfection assays in HeLa cells of various vimentin 5'-end promoter sequences and mutants thereof precisely defined two regulatory elements, a negative element and an adjoining positive acting element. Band shift assays, UV cross-linking, and Southwesternblot analysis confirm that the silencer element specifically binds a protein. Several lines of evidence show that ZBP-89, a zinc finger, Kruppel-likerepressor protein is vimentin's silencer element binding factor. Co-immunoprecipitation and DNA affinity chromatography prove that: Sp1 heterodimerizes with ZBP-89 when bound to the silencer element to yield a DNA-protein complex whose mobility is indistinguishable from that displayed by HeLa nuclear extract in band shift assays.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:24:11