Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Comparison of orthogonal and collinear geometric approaches for design of a laboratory constructed diode laser induced fluorescence detector for capillary electrophoresis
Autore:
Nagaraj, S; Karnes, HT;
Indirizzi:
Virginia Commonwealth Univ, Dept Pharmaceut, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA
Titolo Testata:
INSTRUMENTATION SCIENCE & TECHNOLOGY
fascicolo: 2, volume: 28, anno: 2000,
pagine: 119 - 129
SICI:
1073-9149(2000)28:2<119:COOACG>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRECOLUMN DERIVATIZATION; CYANINE DYE; MICROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Engineering, Computing & Technology
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Karnes, HT Virginia Commonwealth Univ, Dept Pharmaceut, Richmond, VA 23298USA Virginia Commonwealth Univ Richmond VA USA 23298 VA 23298 USA
Citazione:
S. Nagaraj e H.T. Karnes, "Comparison of orthogonal and collinear geometric approaches for design of a laboratory constructed diode laser induced fluorescence detector for capillary electrophoresis", INSTR SCI T, 28(2), 2000, pp. 119-129

Abstract

This work describes the design and construction of two sensitive fluorescence detectors for capillary electrophoresis employing a semiconductor diodelaser as the light source. The two systems employed different geometric approaches - an orthogonal design and a collinear design, for collection of the fluorescence. The systems were evaluated using Cy5.29 carboxylic acid, afar-red dicarbocyanine dye. A limit of detection (LOD), calculated as three times the standard deviation of the blank signal, of 7.4 x 10(-11)M was obtained using the orthogonal system. The precision and accuracy of the assay determined using the orthogonal system were within 10% RSD and 10 % DFN (deviation from nominal) respectively. The collinear design yielded a LOD of 1.2 x 10(-12) M.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/05/20 alle ore 14:34:46