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Titolo:
Metabolism of the dorsal cochlear nucleus in rat brain slices
Autore:
Zheng, L; Godfrey, DA; Waller, HJ; Godfrey, TG; Chen, K; Kong, W;
Indirizzi:
Med Coll Ohio, Dept Otolaryngol Head & Neck Surg, Toledo, OH 43614 USA MedColl Ohio Toledo OH USA 43614 Head & Neck Surg, Toledo, OH 43614 USA
Titolo Testata:
HEARING RESEARCH
fascicolo: 1-2, volume: 143, anno: 2000,
pagine: 115 - 129
SICI:
0378-5955(200005)143:1-2<115:MOTDCN>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-CONCENTRATIONS; HIPPOCAMPAL SLICES; GUINEA-PIG; PROTEIN-SYNTHESIS; IN-VITRO; CEREBROSPINAL-FLUID; SYNAPTIC TRANSMISSION; INVITRO HIPPOCAMPUS; PREPARATIVE METHODS; GLUTAMATE RELEASE;
Keywords:
adenosine triphosphate; choline acetyltransferase; acetylcholinesterase; amino acid; malate dehydrogenase; high performance liquid chromatography;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
86
Recensione:
Indirizzi per estratti:
Indirizzo: Godfrey, DA Med Coll Ohio, Dept Otolaryngol Head & Neck Surg, 3065 Arlington Ave, Toledo, OH 43614 USA Med Coll Ohio 3065 Arlington Ave Toledo OH USA43614 43614 USA
Citazione:
L. Zheng et al., "Metabolism of the dorsal cochlear nucleus in rat brain slices", HEARING RES, 143(1-2), 2000, pp. 115-129

Abstract

In vitro brain slices of the cochlear nucleus have been used for electrophysiological and pharmacological studies. More information is needed about the extent to which the slice resembles in vivo tissue, since this affects the interpretation of results obtained from slices. In this study, some chemical parameters of the dorsal cochlear nucleus (DCN) in rat brain slices were measured and compared to the in vivo state. The activities of malate dehydrogenase and lactate dehydrogenase were reduced in some DCN layers of incubated slices compared to in vivo brain tissue. The activities of choline acetyltransferase and acetylcholinesterase were increased or unchanged in DCN layers of slices. Adenosine triphosphate (ATP) concentrations for in vivorat DCN were similar to those of cerebellar cortex. Compared with in vivo values, ATP concentrations were decreased in the DCN of brain slices, especially in the deep layer. Vibratome-cut slices had lower ATP levels than chopper-cut slices. Compared with the in vivo data, there were large losses ofaspartate, glutamate, glutamine, gamma-aminobutyrate and taurine from incubated slices. These amino acid changes within the slices correlated with the patterns of release from the slices. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:20:51