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Titolo:
ATP synthesis during low-flow ischemia - Influence of increased glycolyticsubstrate
Autore:
Cave, AC; Ingwall, JS; Friedrich, J; Liao, RL; Saupe, KW; Apstein, CS; Eberli, FR;
Indirizzi:
Boston Univ, Sch Med, Whitaker Cardiovasc Inst, Cardiac Muscle Res Lab, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 diac Muscle Res Lab, Boston, MA 02118 USA Brigham & Womens Hosp, Dept Med, Div Cardiovasc, NMR Lab Physiol Chem, Boston, MA 02115 USA Brigham & Womens Hosp Boston MA USA 02115 siol Chem, Boston, MA 02115 USA
Titolo Testata:
CIRCULATION
fascicolo: 17, volume: 101, anno: 2000,
pagine: 2090 - 2096
SICI:
0009-7322(20000502)101:17<2090:ASDLI->2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE MYOCARDIAL-INFARCTION; GLUCOSE-INSULIN-POTASSIUM; DIASTOLIC DYSFUNCTION; CONTRACTILE FAILURE; RAT-HEART; TRIAL; REPERFUSION; METABOLISM; HYPOXIA; THERAPY;
Keywords:
creatine kinase; glucose; insulin; ischemia; metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Apstein, CS 715 Albany St,Room X720, Boston, MA 02118 USA 715 Albany St,Room X720 Boston MA USA 02118 ton, MA 02118 USA
Citazione:
A.C. Cave et al., "ATP synthesis during low-flow ischemia - Influence of increased glycolyticsubstrate", CIRCULATION, 101(17), 2000, pp. 2090-2096

Abstract

Background-Our goals were to (1) simulate the degree of low-flow ischemia and mixed anaerobic and aerobic metabolism of an acutely infarcting region;(2) define changes in anaerobic glycolysis, oxidative phosphorylation, andthe creatine kinase (CK) reaction velocity; and (3) determine whether and how increased glycolytic substrate alters the energetic profile, function, and recovery of the ischemic myocardium in the isolated blood-perfused rat heart. Methods and Results-Hearts had 60 minutes of low-flow ischemia (10% of baseline coronary flow) and 30 minutes of reperfusion with either control or high glucose and insulin (G+I) as substrate. In controls, during ischemia, rate-pressure product and oxygen consumption decreased by 84%. CK velocity decreased by 64%; ATP and phosphocreatine (PCr) concentrations decreased by 51% and 63%, respectively; inorganic phosphate (P-i) concentration increased by 300%; and free [ADP] did not increase. During ischemia, relative to controls, the G+I group had similar CK velocity, oxygen consumption, and tissue acidosis but increased glycolysis, higher [ATP] and [PCr], and lower [P-i] and therefore had a greater free energy yield from ATP hydrolysis. Ischemic systolic and diastolic function and postischemic recovery were better. Conclusions-During low-flow ischemia simulating an acute myocardial infarction region, oxidative phosphorylation accounted for 90% of ATP synthesis. The CK velocity fell by 66%, and CK did not completely use available PCr toslow ATP depletion. G+I, by increasing glycolysis, slowed ATP depletion, maintained lower [P-i], and maintained a higher free energy from ATP hydrolysis. This improved energetic profile resulted in better systolic and diastolic function during ischemia and reperfusion. These results support the clinical use of G+I in acute MI.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:46:16