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Titolo:
Regulation of intracellular pH by bovine intervertebral disc cells
Autore:
Razaq, S; Urban, JPG; Wilkins, RJ;
Indirizzi:
Univ Oxford, Physiol Lab, Oxford OX1 3PT, England Univ Oxford Oxford England OX1 3PT Physiol Lab, Oxford OX1 3PT, England
Titolo Testata:
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 1-2, volume: 10, anno: 2000,
pagine: 109 - 115
SICI:
1015-8987(2000)10:1-2<109:ROIPBB>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTICULAR-CARTILAGE; CHONDROCYTES; EXCHANGE;
Keywords:
intervertebral disc; intracellular pH; BCECF; Na+ x H+ exchange;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Wilkins, RJ Univ Oxford, Physiol Lab, Parks Rd, Oxford OX1 3PT, England Univ Oxford Parks Rd Oxford England OX1 3PT OX1 3PT, England
Citazione:
S. Razaq et al., "Regulation of intracellular pH by bovine intervertebral disc cells", CELL PHYS B, 10(1-2), 2000, pp. 109-115

Abstract

Extracellular acidity is an important determinant of intervertebral disc matrix turnover, possibly exerting effects through changes of intracellular pH (pH(i)). There is, however, little information concerning the ways in which these cells regulate their pH(i), Fluorimetric techniques have been used in the present study to measure pH(i) in isolated intervertebral disc cells, and to characterise the membrane transport pathways by which it is regulated. Nucleus pulposus cells were obtained from bovine intervertebral discs by standard enzymatic digestion techniques, and loaded with the pH-sensitive fluoroprobe BCECF. Resting pH(i) was approximately 6.7 for cells suspended in either HEPES-buffered (HBS) or CO2/HCO3--buffered (BBS) media. Intrinsic buffering capacity was approximately 19 mM pH unit(-1) in HBS and was increased when cells were suspended in BBS. A combination of ion substitution and inhibitor studies for cells at steady-state pH. or acidified by exposure to NH4Cl revealed that in HBS Na+ x H+ exchange and an H+-ATPase extrude acid from these cells. Only one of these two systems, the Na+ x H+ exchanger, exhibited a sensitivity to pH(i), identifying it as the regulator of pH(i) under these conditions. In BBS, an additional pathway which was dependent on extracellular Na+, extracellular HCO3- and intracellular Cl- was detected. These properties are consistent with the four ion HCO3--dependent transporter, although the cation-rich, anion-poor extracellular matrix of the intervertebral disc means that such a pathway has only a marginal role indisc cell pH(i) regulation. Copyright (C) 2000 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 00:55:11