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Titolo:
Diadenosine polyphosphates and atrial natriuretic peptide are antiproliferative in rat mesangial cells
Autore:
Schulte, EA; Saleh, H; Schlatter, E;
Indirizzi:
Univ Munster, Med Poliklin, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 Poliklin, D-48149 Munster, Germany
Titolo Testata:
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 1-2, volume: 10, anno: 2000,
pagine: 57 - 64
SICI:
1015-8987(2000)10:1-2<57:DPAANP>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR; MATRIX ACCUMULATION; INTRACELLULAR PH; NA+/H+ EXCHANGE; ANGIOTENSIN-II; PROLIFERATION; EXPRESSION; AGENTS; ATP;
Keywords:
mesangial cells; rat; proliferation; atrial natriuretic peptide; diadenosine polyphosphates; PDGF; thapsigargin; H-3-thymidine incorporation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Schlatter, E Univ Munster, Med Poliklin, Domagkstr 3A, D-48149 Munster, Germany Univ Munster Domagkstr 3A Munster Germany D-48149 r, Germany
Citazione:
E.A. Schulte et al., "Diadenosine polyphosphates and atrial natriuretic peptide are antiproliferative in rat mesangial cells", CELL PHYS B, 10(1-2), 2000, pp. 57-64

Abstract

Modulation of cell proliferation by vasoactive hormones and growth factorsinvolves also changes in the activity of pH-regulatory transporters. In a preceeding paper (1) we examined the influence of such factors on cellular pH(i). Here the influence of the same factors, diadenosine polyphosphates (ApnA), atrial natriuretic peptide, the growth factor PDGF and the Ca2+-ATPase inhibitor thapsigargin, on proliferation of cultured rat mesangial cellswas examined by quantification of [H-3]-thymidine incorporation. Mesangialcells were synchronised and growth reduced (0.5% FCS for 24 h) before experiments were started. Incubation with Ap3A, Ap4A, Ap5A or Ap6A (all 10 mu M) for 24 h all reduced cell proliferation by 30 to 45%. At 0.1 and 1 mu M the effects of Ap4A, Ap5A and Ap6A did not reach significance. The antimitogenic effect of Ap5A was not significantly different when cells were incubated for 24, 48 or 72 h. In addition there was no significant difference between the antiproliferative effect of Ap5A in cells of the second, sixth or thirteenth passage. The growth factor PDGF-BB (0.25 nM) resulted in an approximately 3-fold increase in [H-3]-thymidine incorporation. This increase inproliferation could be significantly reduced by coincubation with 10 CIM Ap5A, The mitogenic effect of PDGF was completely abolished in the presence of the Ca2+-ATPase inhibitor thapsigargin (1 nM), which also significantly reduced basal cell proliferation by approximately 40%. Incubation of mesangial cells with 10 nM ANP for 24 h reduced basal [H-3]-thymidine incorporation slightly by approximately 20% and decreased the PDGF-induced stimulation. The antimitogenic effects of these agonists is especially pronounced when cells are stimulated. Copyright (C) 2000 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 14:25:32