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Titolo:
A modification of Mayer's tannic acid-ferric chloride staining method for demonstrating cellular membranous systems for light microscopy
Autore:
Matsui, S; Hatanaka, K; Watanabe, H; Asano, Y; Yamagata, H; Maruyama, T;
Indirizzi:
Shionogi & Co Ltd, Med Advisor Off, Fukushima Ku, Osaka 5530002, Japan Shionogi & Co Ltd Osaka Japan 5530002 Fukushima Ku, Osaka 5530002, Japan Shionogi & Co Ltd, Dev Res Labs, Toyonaka, Osaka 5610825, Japan Shionogi &Co Ltd Toyonaka Osaka Japan 5610825 naka, Osaka 5610825, Japan
Titolo Testata:
BIOTECHNIC & HISTOCHEMISTRY
fascicolo: 1, volume: 75, anno: 2000,
pagine: 33 - 40
SICI:
1052-0295(200001)75:1<33:AMOMTA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CADMIUM-METALLOTHIONEIN CDMT; PROTEIN; ACCUMULATION;
Keywords:
ferric chloride; light microscopy; membranes; paraffin-embedment; proximal convoluted tubule; renal tubular segments; tannic acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Hatanaka, K Shionogi & Co Ltd, Med Advisor Off, Fukushima Ku, 5-12-4 Sagisu, Osaka 5530002, Japan Shionogi & Co Ltd 5-12-4 Sagisu Osaka Japan 5530002 02, Japan
Citazione:
S. Matsui et al., "A modification of Mayer's tannic acid-ferric chloride staining method for demonstrating cellular membranous systems for light microscopy", BIOTECH HIS, 75(1), 2000, pp. 33-40

Abstract

To observe cellular membranous systems under a light microscope, we modified Mayer's tannic acid-ferric chloride stain method by adding a treatment with hematoxylin after the original procedure. We used the modified tannic acid-ferric chloride (MTA-Fe) stain method to examine kidneys, liver, heart,trachea, epididymides and other organs of rats and dogs. The MTA-Fe stain clearly demonstrated the basement membrane, brush border, basolateral invaginations and cell processes in the kidneys which enabled easy differentiation of the S1 and S3 segments of proximal convoluted tubules. Our technique also demonstrated hepatic cell membranes and bile canaliculi in the liver, cross striations and longitudinal traveling of myofibrils in the heart, cilia of the epithelial cells in the trachea, and stereocilia and terminal bars in the epididymis. The MTA-Fe stain is a convenient method to visualize cellular membranous systems even for light microscopy. The stain has the advantages of using no toxic materials, simple and easy technique, little variation of staining results, and little fading for several months after staining.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:52:02