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Titolo:
Enzymatic production of trans-4-hydroxy-L-proline by regio- and stereospecific hydroxylation of L-proline
Autore:
Shibasaki, T; Mori, H; Ozaki, A;
Indirizzi:
Kyowa Hakko Kogyo Co Ltd, Tokyo Res Labs, Tokyo 1948533, Japan Kyowa HakkoKogyo Co Ltd Tokyo Japan 1948533 Labs, Tokyo 1948533, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 4, volume: 64, anno: 2000,
pagine: 746 - 750
SICI:
0916-8451(200004)64:4<746:EPOTBR>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; MICROORGANISMS; 4-HYDROXYLASE; BIOSYNTHESIS; PURIFICATION; PROMOTER; CLONING; BLOCK;
Keywords:
hydroxyproline; proline; bioconversion; proline 4-hydroxylase; 2-oxoglutarate-dependent dioxygenase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Shibasaki, T Kyowa Hakko Kogyo Co Ltd, Tech Res Labs, 1-1 Kyowamachi, Yamaguchi 7478522, Japan Kyowa Hakko Kogyo Co Ltd 1-1 Kyowamachi Yamaguchi Japan 7478522
Citazione:
T. Shibasaki et al., "Enzymatic production of trans-4-hydroxy-L-proline by regio- and stereospecific hydroxylation of L-proline", BIOS BIOT B, 64(4), 2000, pp. 746-750

Abstract

A proline 4-hydroxylase gene, which was cloned from Dactylosporangium sp. RH1, was overexpressed in Escherichia coli W1485 on a plasmid under a tryptophan tandem promoter after the codon usage of the 5' end of the gene was optimized. The proline 4-hydroxylase activity was 1600-fold higher than thatin Dactylosporangium sp, RH1. trans-4-Hydroxy-L-proline(Hyp) was produced and accumulated to 41 g/L (87% yield from L-proline) in 100 h when the recombinant E. coli was cultivated in a medium containing L-proline and glucose. 2-Oxoglutarate, which is necessary for the hydroxylation of L-proline by proline 4-hydroxylase, was apparently supplied from glucose through the cellular metabolic pathway. The putA mutant of W1485, which is not able to degrade L-proline, has allowed the quantitative conversion of L-proline to Hyp. The formation of other isomers of hydroxyproline was not observed. Productivity of Hyp was almost the same in a larger-scale culture. The method of manufacturing Hyp from L-proline was established.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:40:27