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Titolo:
Efficient translesion replication past oxaliplatin and cisplatin GpG adducts by human DNA polymerase eta
Autore:
Vaisman, A; Masutani, C; Hanaoka, F; Chaney, SG;
Indirizzi:
Univ N Carolina, Lineberger Comprehens Canc Ctr, Dept Biochem & Biophys, Sch Med, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599h Med, Chapel Hill, NC 27599 USA Osaka Univ, Inst Mol & Cellular Biol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 ar Biol, Suita, Osaka 5650871, Japan
Titolo Testata:
BIOCHEMISTRY
fascicolo: 16, volume: 39, anno: 2000,
pagine: 4575 - 4580
SICI:
0006-2960(20000425)39:16<4575:ETRPOA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
XERODERMA-PIGMENTOSUM; THYMINE DIMER; CELL-LINES; IN-VITRO; BYPASS; BETA; GENE; CIS-DIAMMINEDICHLOROPLATINUM(II); LESION; SENSITIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Chaney, SG Univ N Carolina, Lineberger Comprehens Canc Ctr, Dept Biochem &Biophys, Sch Med, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 el Hill, NC 27599 USA
Citazione:
A. Vaisman et al., "Efficient translesion replication past oxaliplatin and cisplatin GpG adducts by human DNA polymerase eta", BIOCHEM, 39(16), 2000, pp. 4575-4580

Abstract

Platinum anticancer agents form bulky DNA adducts which are thought to exert their cytotoxic effect by blocking DNA replication. Translesion synthesis, one of the pathways of postreplication repair, is thought to account forsome resistance to DNA damage and much of the mutagenicity of bulky DNA adducts in dividing cells. Oxaliplatin has been shown to be effective in cisplatin-resistant cell lines and less mutagenic than cisplatin in the Ames assay. We have shown that the eukaryotic DNA polymerases yeast pol zeta, human pol beta, and human pol gamma bypass oxaliplatin-GG adducts more efficiently than cisplatin-GG adducts. Human pol eta, a product of the XPV gene, has been shown to catalyze efficient translesion synthesis past cis,syn-cyclobutane pyrimidine dimers. In the present study we compared translesion synthesis past different Pt-GG adducts by human pol eta. Our data show that, similar to other eukaryotic DNA polymerases, pol eta bypasses oxaliplatin-GG adducts more efficiently than cisplatin-GG adducts. However, pol eta-catalyzed translesion replication past Pt-DNA adducts was more efficient and lessaccurate than that seen for previously tested polymerases. We show that the efficiency and fidelity of translesion replication Fast Pt-DNA adducts appear to be determined by both the structure of the adduct and the DNA polymerase active site.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:22:21